The elimination of O-linked glycans from glycoproteins under non-reducing conditions.

Glycoconjugate journal ·Vol. 11 ·No. 2 ·1994-04-00 ·Pages 163-7

Cooper CA, Packer NH, Redmond JW

Abstract

A simple procedure is described for the elimination of O-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive beta-elimination of O-glycans.

MeSH Terms

Acetone Animals Carbohydrates/analysis,chemistry Cattle Chromatography, Gel Chromatography, High Pressure Liquid Ethylamines Glycoproteins/chemistry Hydrazines Indicators and Reagents Mucins/chemistry,isolation & purification Oxidation-Reduction Polysaccharides/chemistry,isolation & purification Solutions Submandibular Gland

Chemicals

Carbohydrates Ethylamines Glycoproteins Hydrazines Indicators and Reagents Mucins Polysaccharides Solutions Acetone hydrazine triethylamine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cooper C A

School of Chemistry, Macquarie University, North Ryde, NSW, Australia.

Packer N H

Redmond J W

References (12)

12 references, click to expand

Mild alkaline borohydride treatment of glycoproteins-a method for liberating both N- and O-linked carbohydrate chains.
Anal Biochem. 1982 Jan 15;119(2):351-9 PMID: 7041698
The effect of mild alkali and alkaline borohydride on the carbohydrate and peptide moieties of fetuin.
Biochem Soc Trans. 1984 Aug;12(4):607-10 PMID: 6208065
Purification and properties of an endo-alpha-N-acetyl-D-galactosaminidase from Diplococcus pneumoniae.
J Biol Chem. 1977 Dec 10;252(23):8609-14 PMID: 21877
The effect of alkaline borohydride treatment on N-linked carbohydrates of glycoproteins.
Glycoconj J. 1989;6(1):45-56 PMID: 2535476
Reductive cleavage of Xaa-proline peptide bonds by mild alkaline borohydride treatment employed to release O-glycosidically linked carbohydrate units of glycoproteins.
Arch Biochem Biophys. 1984 Aug 1;232(2):699-706 PMID: 6380410
Hydrazinolysis of asparagine-linked sugar chains to produce free oligosaccharides.
Methods Enzymol. 1982;83:263-8 PMID: 7098932
STUDIES ON GLYCOPROTEINS. XI. THE O-GLYCOSIDIC LINKAGE OF N-ACETYLGALACTOSAMINE TO SERYL AND THREONYL RESIDUES IN OVINE SUBMAXILLARY GLAND GLYCOPROTEIN.
Biochim Biophys Acta. 1965 Mar 1;101:67-82 PMID: 14329290
Hydrazinolysis-N-reacetylation of glycopeptides and glycoproteins. Model studies using 2-acetamido-1-N-(L-aspart-4-oyl)-2-deoxy-beta-D-glucopyranosy lamine.
Carbohydr Res. 1985 Nov 15;144(1):1-12 PMID: 4075333
Action of alkali on bovine and ovine submaxillary mucins.
J Biol Chem. 1967 Sep 10;242(17):3776-81 PMID: 6037543
Use of hydrazine to release in intact and unreduced form both N- and O-linked oligosaccharides from glycoproteins.
Biochemistry. 1993 Jan 19;32(2):679-93 PMID: 8422375
Separation and identification of O-linked oligosaccharides derived from glycoproteins by high-pH anion-exchange chromatography.
Anal Biochem. 1993 May 15;211(1):72-80 PMID: 7686723
High performance anion exchange chromatography of reduced oligosaccharides from sialomucins.
Glycoconj J. 1991 Dec;8(6):493-8 PMID: 1823623

Article Info

Journal

Glycoconjugate journal

Abbr.

Glycoconj J

ISSN

0282-0080

DOI

10.1007/BF00731156

Published

1994-04-00

Pages

163-7

Language

English

Region

United States

NLM ID

8603310

Subset

External Links

PubMed source DOI: 10.1007/BF00731156 View journal details

Analysis Services

The elimination of O-linked glycans from glycoproteins under non-reducing conditions.

Contact

WeChat Official Account