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PMID: 781677 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Detergent-soluble HLA antigens contain a hydrophilic region at the COOH-terminus and a penultimate hydrophobic region.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 7 ·1976-07-00 ·Pages 2481-5

Springer TA, Strominger JL

Abstract

Purified, detergent-soluble HLA antigens (p44,12) are composed of a glycoprotein of molecular weight 44,000 (p44) and a peptide of molecular weight 12,000 (p12), beta2-microglobulin. Upon digestion with papain, p44,12 is converted to p39,12, then to p34,12, which retains antigenic activity. The NH2-terminal amino acid sequences of p34 and p44 are identical. p44, p39, and p34 were purified, and comparison of their amino acid compositions showed that the COOH-terminal peptide removed by the first papain cleavage is hydrophilic and contains cysteine that can be alkylated after mild reduction. The penultimate COOH-terminal peptide removed by the second papain cleavage is hydrophobic, and presumably anchors HLA antigens to the membrane. This correlates with the observation that p44,12 and p39,12 bind detergent, while p34,12 does not. The orientation and integration of HLA antigens in the lymphocyte membrane were thus defined, and the structure suggests that HLA antigens span the plasma membrane.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Cell Membrane/immunology Cysteine/analysis HLA Antigens/analysis Histocompatibility Antigens/analysis Humans Models, Biological Papain Solubility beta 2-Microglobulin/isolation & purification
Chemicals
Amino Acids HLA Antigens Histocompatibility Antigens beta 2-Microglobulin Papain Cysteine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Springer T A
Strominger J L
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.7.2481
Published
1976-07-00
Pages
2481-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430616
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.7.2481 PMC Full Text View journal details
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