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PMID: 7823904 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Abscisic acid-dependent and -independent regulation of gene expression by progressive drought in Arabidopsis thaliana.

Molecular & general genetics : MGG ·Vol. 246 ·No. 1 ·1995-01-06 ·Pages 10-8

Gosti F, Bertauche N, Vartanian N, Giraudat J

Abstract

Four clones corresponding to Arabidopsis thaliana transcripts regulated by progressive drought stress were isolated. Abundance of the AtDi8, AtDi19 and AtDi21 mRNAs increased in both roots and leaves during progressive drought. The AtDr4 mRNA was expressed in a root-specific manner in regularly watered plants, and became undetectable under drought conditions. In all cases, the drought-induced modifications of mRNA abundance could be reversed by subsequent rehydration. The predicted AtDr4 protein displays extensive similarity to various members of the Künitz protein family, suggesting that AtDr4 might be a root-specific protease inhibitor. Of these four genes, only AtDi8 and AtDi21 responded to an exogenous supply of abscisic acid (ABA). Analysis of the ABA-deficient aba mutant demonstrated that endogenous ABA indeed participates in the drought regulation of these two transcripts. This ABA-dependent response was differentially affected in the various classes of ABA-insensitive Arabidopsis mutants. The AtDi19 and AtDr4 mRNAs both responded to drought in an ABA-independent manner, but at distinct thresholds of the progressive drought stress. Regulation of these four target genes by progressive drought stress thus appears to be mediated by at least three distinct signals, only one of which is ABA.

Related Genes
AtDi19 AtDi21 AtDi8 AtDr4
MeSH Terms
Abscisic Acid/physiology Adaptation, Biological/genetics Amino Acid Sequence Arabidopsis/enzymology,genetics,physiology Arabidopsis Proteins Desiccation Down-Regulation Gene Expression Regulation, Plant Genes, Plant Molecular Sequence Data Plant Proteins/chemistry,genetics Plant Roots/enzymology Protease Inhibitors RNA, Messenger/analysis RNA, Plant/analysis Sequence Alignment Sequence Homology, Amino Acid
Chemicals
Arabidopsis Proteins AtDr4 protein, Arabidopsis Plant Proteins Protease Inhibitors RNA, Messenger RNA, Plant Abscisic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gosti F
Institut des Sciences Végétales, Centre National de la Recherche Scientifique UPR 40, Gif-sur-Yvette Cedex, France.
Bertauche N
Vartanian N
Giraudat J
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00290128
Published
1995-01-06
Pages
10-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X78584, X78585, X78586
External Links
PubMed source DOI: 10.1007/BF00290128
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