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PMID: 7828811 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of G1 checkpoint control in the yeast Saccharomyces cerevisiae following exposure to DNA-damaging agents.

Genetics ·Vol. 138 ·No. 2 ·1994-10-00 ·Pages 271-81

Siede W, Friedberg AS, Dianova I, Friedberg EC

Abstract

The delay of S-phase following treatment of yeast cells with DNA-damaging agents is an actively regulated response that requires functional RAD9 and RAD24 genes. An analysis of cell cycle arrest indicates the existence of (at least) two checkpoints for damaged DNA prior to S-phase; one at START (a G1 checkpoint characterized by pheromone sensitivity of arrested cells) and one between the CDC4- and CDC7-mediated steps (termed the G1/S checkpoint). When a dna1-1 mutant (that affects early events of replicon initiation) also carries a rad9 deletion mutation, it manifests a failure to arrest in G1/S following incubation at the restrictive temperature. This failure to execute regulated G1/S arrest is correlated with enhanced thermosensitivity of colony-forming ability. In an attempt to characterize the signal for RAD9 gene-dependent G1 and G1/S cell cycle arrest, we examined the influence of the continued presence of unexcised photoproducts. In mutants defective in nucleotide excision repair, cessation of S-phase was observed at much lower doses of UV radiation compared to excision-proficient cells. However, this response was not RAD9-dependent. We suggest that an intermediate of nucleotide excision repair, such as DNA strand breaks or single-stranded DNA tracts, is required to activate RAD9-dependent G1 and G1/S checkpoint controls.

Related Genes
CDC4 CDC7 RAD24 RAD9 dna1-1
MeSH Terms
Cell Cycle/genetics,radiation effects Cell Cycle Proteins DNA Damage DNA-Binding Proteins Endodeoxyribonucleases Flow Cytometry Fungal Proteins/genetics,metabolism G1 Phase/genetics,radiation effects Gene Deletion Genes, Fungal Genotype Intracellular Signaling Peptides and Proteins Mating Factor Peptide Biosynthesis Peptides Pheromones/biosynthesis Restriction Mapping Saccharomyces cerevisiae/genetics,growth & development,radiation effects Saccharomyces cerevisiae Proteins Species Specificity Transcription, Genetic
Chemicals
Cell Cycle Proteins DNA-Binding Proteins Fungal Proteins Intracellular Signaling Peptides and Proteins Peptides Pheromones RAD24 protein, S cerevisiae Saccharomyces cerevisiae Proteins RAD2 protein, S cerevisiae rad9 protein Mating Factor Endodeoxyribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Siede W
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235.
Friedberg A S
Dianova I
Friedberg E C
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/138.2.271
Published
1994-10-00
Pages
271-81
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206146
Subset
IM
Grants
NCI NIH HHS · CA12428 · United States
External Links
PubMed source DOI: 10.1093/genetics/138.2.271 PMC Full Text View journal details
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