Abstract
Capsid-targeted viral inactivation is an antiviral strategy in which toxic fusion proteins are targeted to virions, where they inhibit viral multiplication by destroying viral components. These fusion proteins consist of a virion structural protein moiety and an enzymatic moiety such as a nuclease. Such fusion proteins can severely inhibit transposition of yeast retrotransposon Ty1, an element whose transposition mechanistically resembles retroviral multiplication. We demonstrate that expression of a murine retrovirus capsid-staphylococcal nuclease fusion protein inhibits multiplication of the corresponding murine leukemia virus by 30- to 100-fold. Staphylococcal nuclease is apparently inactive intracellularly and hence nontoxic to the host cell, but it is active extracellularly because of its requirement for high concentrations of Ca2+ ions. Virions assembled in and shed from cells expressing the fusion protein contain very small amounts of intact viral RNA, as would be predicted for nuclease-mediated inhibition of viral multiplication.
MeSH Terms
3T3 Cells
Animals
Antiviral Agents/metabolism
Capsid/metabolism
Gene Products, gag/genetics,metabolism,pharmacology
Leukemia Virus, Murine/growth & development
Mice
Micrococcal Nuclease/genetics,metabolism,pharmacology
RNA, Viral/metabolism
Recombinant Fusion Proteins/metabolism,pharmacology
Virion/growth & development
Virus Replication
Chemicals
Antiviral Agents
Gene Products, gag
RNA, Viral
Recombinant Fusion Proteins
Micrococcal Nuclease
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Natsoulis G
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Seshaiah P
Federspiel M J
Rein A
Hughes S H
Boeke J D
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