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PMID: 7835321 Published · ppublish English Journal Article

A fluorescence depolarization study of the orientational distribution of crossbridges in muscle fibres.

European biophysics journal : EBJ ·Vol. 23 ·No. 5 ·1994-00-00 ·Pages 369-78

van der Heide UA, Rem OE, Gerritsen HC, de Beer EL, Schiereck P, Trayer IP, Levine YK

Abstract

A fluorescence depolarization study of the orientational distribution of crossbridges in dye-labelled muscle fibres is presented. The characterization of this distribution is important since the rotation of crossbridges is a key element in the theory of muscle contraction. In this study we exploited the advantages of angle-resolved experiments to characterize the principal features of the orientational distribution of the crossbridges in the muscle fibre. The directions of the transition dipole moments in the frame of the dye and the orientation and motion of the dye relative to the crossbridge determined previously were explicitly incorporated into the analysis of the experimental data. This afforded the unequivocal determination of all the second and fourth rank order parameters. Moreover, this additional information provided discrimination between different models for the orientational behaviour of the crossbridges. Our results indicate that no change of orientation takes place upon a transition from rigor to relaxation. The experiments, however, do no rule out a conformational change of the myosin S1 during the transition.

MeSH Terms
Animals Fluorescent Dyes Models, Biological Models, Theoretical Muscle Contraction Muscle Fibers, Skeletal/physiology,ultrastructure Muscle, Skeletal/physiology,ultrastructure Naphthalenesulfonates Rabbits Scattering, Radiation Spectrometry, Fluorescence
Chemicals
Fluorescent Dyes Naphthalenesulfonates 1,5-I-AEDANS
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
van der Heide U A
Debye Institute, Department of Molecular Biophysics, University of Utrecht, Buys Ballot Laboratory, The Netherlands.
Rem O E
Gerritsen H C
de Beer E L
Schiereck P
Trayer I P
Levine Y K
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Article Info
Journal
European biophysics journal : EBJ
Abbr.
Eur Biophys J
ISSN
0175-7571
DOI
10.1007/BF00188661
Published
1994-00-00
Pages
369-78
Language
English
Region
Germany
NLM ID
8409413
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00188661 View journal details
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