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PMID: 7835957 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Protection against tuberculosis by passive transfer with T-cell clones recognizing mycobacterial heat-shock protein 65.

Immunology ·Vol. 83 ·No. 3 ·1994-11-00 ·Pages 341-6

Silva CL, Silva MF, Pietro RC, Lowrie DB

Abstract

We have previously shown that mice vaccinated by injection with J774 macrophage-like tumour cells that expressed Mycobacterium leprae heat-shock protein (hsp) 65 as a transgene had acquired a remarkably high degree of protection against subsequent challenge with virulent M. tuberculosis. We show here that antigen-specific T cells cloned from spleens of such vaccinated animals can transfer a high level of protection to non-vaccinated recipients. The most efficient cells were of T-cell receptor (TCR) alpha beta+ and CD4- CD8+ type and specifically lysed mycobacteria-infected macrophages. These findings are consistent with the importance for protective immunity of engaging the endogenous antigen-presenting pathway to bias the immune response towards a cytolytic action against a mycobacterial antigen that is expressed at the surface of infected macrophages. TCR gamma delta+ and TCR alpha beta+ cells interacted synergistically.

MeSH Terms
Animals Antigen-Presenting Cells/immunology Antigens, Bacterial/administration & dosage Bacterial Proteins CD8-Positive T-Lymphocytes/immunology Chaperonin 60 Chaperonins/immunology Heat-Shock Proteins/immunology Immunization, Passive Mice Mice, Inbred BALB C Mycobacterium leprae/immunology Receptors, Antigen, T-Cell, alpha-beta/immunology Receptors, Antigen, T-Cell, gamma-delta/immunology T-Lymphocytes/immunology Tuberculosis/prevention & control
Chemicals
Antigens, Bacterial Bacterial Proteins Chaperonin 60 Heat-Shock Proteins Receptors, Antigen, T-Cell, alpha-beta Receptors, Antigen, T-Cell, gamma-delta heat-shock protein 65, Mycobacterium Chaperonins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Silva C L
Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
Silva M F
Pietro R C
Lowrie D B
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1994-11-00
Pages
341-6
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1415049
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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