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PMID: 785024 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Membrane protein biosynthesis in bacteriophage BF23-infected Escherichia coli.

Journal of virology ·Vol. 19 ·No. 2 ·1976-08-00 ·Pages 475-89

Billmire EW, Duckworth DH

Abstract

When Escherichia coli is infected with bacteriophage BF23, two new proteins with molecular weights greater than 10,000, as indicated by polyacrylamide gel electrophoresis, are found associated with the cells' membranes. One of these, found associated with both the inner and outer membrane, has a molecular weight of about 55,000 and is regulated by the A1 gene of this phage, a gene found on the spontaneously injected 8% piece of BF23 DNA, DNA that codes for the synthesis of proteins necessary for the injection of the whole phage genome. The other protein, often undetected in whole membrane preparations, is found exclusively associated with the inner membrane. Evidence indicates that this protein is also regulated by the initially injected 8% piece of the DNA.

MeSH Terms
Cell Membrane/metabolism Cell Wall/metabolism Coliphages/growth & development,metabolism DNA, Viral/metabolism Escherichia coli/metabolism Genes Molecular Weight Mutation Viral Proteins/biosynthesis
Chemicals
DNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Billmire E W
Duckworth D H
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22 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.19.2.475-489.1976
Published
1976-08-00
Pages
475-89
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354885
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.19.2.475-489.1976 PMC Full Text View journal details
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