Genetics of borrelidin resistant mutants of Saccharomyces cerivisiae and properties of their threonyl-tRNA-synthetase.

Molecular & general genetics : MGG ·Vol. 147 ·No. 1 ·1976-08-10 ·Pages 39-43

Nass G, Poralla K

Abstract

Twenty-two borrelidin resistant mutants of Saccharomyces cerivisiae were isolated, studied genetically and their threonyl-tRNA-synthetase was investigated. The borrelidin resistant mutants are classified into four groups. In the first group borrelidin resistance is coupled to the gene HOM3 coding for aspartokinase, in the second group to the gene LEU1. The borrelidin resistance in group three and four is not coupled to anyone of the genetic markers tested. Borrelidin resistance exhibited dominant behavior in all mutants except in the mutant of group 4. The properties of the ThrRS of the mutants of group one, two and four was found to be like the ones of the wild types. However the mutants of group three exhibit a structurally altered ThrRS, which is no longer inhibited by borrelidin.

MeSH Terms

Amino Acyl-tRNA Synthetases/metabolism Anti-Bacterial Agents/pharmacology Chromosome Mapping Chromosomes Cyclopentanes/pharmacology Drug Resistance, Microbial Fatty Alcohols/pharmacology Genes Genetic Linkage Mutation Saccharomyces cerevisiae/drug effects,enzymology Threonine-tRNA Ligase/metabolism

Chemicals

Anti-Bacterial Agents Cyclopentanes Fatty Alcohols Amino Acyl-tRNA Synthetases Threonine-tRNA Ligase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Nass G

Poralla K

References (13)

13 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00337933

Published

1976-08-10

Pages

39-43

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00337933

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Genetics of borrelidin resistant mutants of Saccharomyces cerivisiae and properties of their threonyl-tRNA-synthetase.

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