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PMID: 7868239 Published · ppublish English Comparative Study Journal Article

Cholera toxin and Salmonella typhimurium induce different cytokine profiles in the gastrointestinal tract.

Infection and immunity ·Vol. 63 ·No. 3 ·1995-03-00 ·Pages 1134-7

Klimpel GR, Asuncion M, Haithcoat J, Niesel DW

Abstract

Salmonella infection of the gastrointestinal tract (GT) results in fluid secretion and inflammation. In contrast, cholera toxin (CT) induces fluid secretion but no inflammation. Using a murine ligated intestinal loop model, we investigated cytokine production (interleukin-1 [IL-1], IL-2, IL-4, IL-6, IL-10, gamma interferon, and tumor necrosis factor alpha) in the GT following exposure to these agents. Salmonella typhimurium induced a Th1-like cytokine profile in loops obtained from either nonimmune mice or Salmonella-immunized mice. CT induced only IL-6 and IL-10 production in ligated loops from nonimmune mice but induced a Th2-like cytokine profile in ligated loops obtained from CT-immunized mice. These results show that CT and S. typhimurium induce very different cytokine profiles in the GT.

MeSH Terms
Animals Cholera Toxin/immunology Cytokines/analysis,genetics,immunology Enzyme-Linked Immunosorbent Assay Intestines/chemistry,immunology Ligation Mice Mice, Inbred CBA Polymerase Chain Reaction RNA, Messenger/analysis Salmonella typhimurium/immunology
Chemicals
Cytokines RNA, Messenger Cholera Toxin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Klimpel G R
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston 77555-1019.
Asuncion M
Haithcoat J
Niesel D W
References (19)
19 references, click to expand
  1. Generalized systemic and mucosal immunity in mice after mucosal stimulation with cholera toxin.
    J Immunol. 1984 Jun;132(6):2736-41 PMID: 6233359
  2. IL-10 acts on the antigen-presenting cell to inhibit cytokine production by Th1 cells.
    J Immunol. 1991 May 15;146(10):3444-51 PMID: 1827484
  3. Cholera toxin discriminates between T helper 1 and 2 cells in T cell receptor-mediated activation: role of cAMP in T cell proliferation.
    J Exp Med. 1990 Jul 1;172(1):95-103 PMID: 2162906
  4. Generation of interleukin 4 (IL-4)-producing cells in vivo and in vitro: IL-2 and IL-4 are required for in vitro generation of IL-4-producing cells.
    J Exp Med. 1990 Sep 1;172(3):921-9 PMID: 2117636
  5. Production of cytokines by mouse B cells: B lymphomas and normal B cells produce interleukin 10.
    Int Immunol. 1990;2(9):821-32 PMID: 1703785
  6. IL-10 inhibits cytokine production by activated macrophages.
    J Immunol. 1991 Dec 1;147(11):3815-22 PMID: 1940369
  7. Interleukin-6 response of epithelial cell lines to bacterial stimulation in vitro.
    Infect Immun. 1992 Apr;60(4):1295-301 PMID: 1347759
  8. Cholera toxin adjuvant promotes long-term immunological memory in the gut mucosa to unrelated immunogens after oral immunization.
    Immunology. 1992 Mar;75(3):488-92 PMID: 1572696
  9. IL-10 inhibits mitogen-induced T cell proliferation by selectively inhibiting macrophage costimulatory function.
    J Immunol. 1992 May 15;148(10):3133-9 PMID: 1578140
  10. Interferon gamma (IFN-gamma) production by gut-associated lymphoid tissue and spleen following oral Salmonella typhimurium challenge.
    Microb Pathog. 1991 Nov;11(5):347-56 PMID: 1816488
  11. Interleukin-10.
    Annu Rev Immunol. 1993;11:165-90 PMID: 8386517
  12. Tumor necrosis factor-alpha mediates the early pathology in Salmonella infection of the gastrointestinal tract.
    Microb Pathog. 1993 Mar;14(3):217-27 PMID: 8321123
  13. Helper T cell subsets for immunoglobulin A responses: oral immunization with tetanus toxoid and cholera toxin as adjuvant selectively induces Th2 cells in mucosa associated tissues.
    J Exp Med. 1993 Oct 1;178(4):1309-20 PMID: 8376936
  14. Tumor necrosis factor (TNF alpha) regulates intestinal mucus production during salmonellosis.
    Cell Immunol. 1993 Oct 15;151(2):336-44 PMID: 8402940
  15. Enhancing effect of cholera toxin on interleukin-6 secretion by IEC-6 intestinal epithelial cells: mode of action and augmenting effect of inflammatory cytokines.
    Infect Immun. 1993 Nov;61(11):4637-44 PMID: 8406861
  16. Differential cytokine expression by human intestinal epithelial cell lines: regulated expression of interleukin 8.
    Gastroenterology. 1993 Dec;105(6):1689-97 PMID: 8253345
  17. Molecular mediators formed in the small intestine in response to cholera toxin.
    J Diarrhoeal Dis Res. 1993 Dec;11(4):227-34 PMID: 8188995
  18. Functional diversity of T lymphocytes due to secretion of different cytokine patterns.
    FASEB J. 1991 Feb;5(2):171-7 PMID: 1825981
  19. Role of prostaglandins and cAMP in the secretory effects of cholera toxin.
    Science. 1989 Aug 25;245(4920):857-9 PMID: 2549637
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.63.3.1134-1137.1995
Published
1995-03-00
Pages
1134-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173123
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.63.3.1134-1137.1995 PMC Full Text View journal details
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