Home LiteratureArticle Details
PMID: 786880 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Distribution of dengue-2 antigens by electron immunocytochemistry.

Infection and immunity ·Vol. 13 ·No. 6 ·1976-06-00 ·Pages 1699-709

Cardiff RD, Lund JK

Abstract

The distribution of dengue-2 antigens was studied in infected monkey kidney cells (LLC MK2) using an indirect, horseradish peroxidase-conjugated immunoglobulin technique. This procedure allowed both light and electron microscopic examination of serial-step sections of individual cells cut in a plane perpendicular to the monolayer. Both virion and nonvirion antigens were identified on the plasma membrane with this technique. A diffuse cytoplasmic reaction product was also present. The intensity and distribution of the cytoplasmic reaction product was related to disruption of the plasma membrane.

MeSH Terms
Animals Antigens, Viral/analysis Cell Membrane/ultrastructure Dengue Virus/immunology,ultrastructure Fluorescent Antibody Technique Kidney/immunology Mice Microscopy, Electron
Chemicals
Antigens, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cardiff R D
Lund J K
References (12)
12 references, click to expand
  1. Detection of dengue cell-surface antigens by peroxidase-labeled antibodies and immune cytolysis.
    Infect Immun. 1974 Aug;10(2):381-8 PMID: 4136628
  2. Immunoprecipitation analysis of soluble complement-fixing antigens of dengue viruses.
    J Immunol. 1970 Oct;105(4):838-45 PMID: 4097142
  3. Genetic analysis of simian virus 40. I. Description of microtitration and replica-plating techniques for virus.
    Virology. 1970 Aug;41(4):751-60 PMID: 4319784
  4. Cytological localization of Dengue-2 antigens: an immunological study with ultrastructural correlation.
    Infect Immun. 1973 May;7(5):809-16 PMID: 4202963
  5. Human antibody to dengue soluble complement-fixing (SCF) antigens.
    J Immunol. 1973 Dec;111(6):1804-9 PMID: 4201557
  6. Replication of Sindbis virus. IV. Electron microscope study of the insertion of viral glycoproteins into the surface of infected chick cells.
    J Virol. 1974 Aug;14(2):366-74 PMID: 4858787
  7. Membrane-bound proteins of Japanese encephalitis virus-infected chick embryo cells.
    Virology. 1972 May;48(2):360-72 PMID: 5024605
  8. Influence of cell type and virus upon virus-specific immune cytolysis.
    Infect Immun. 1975 Feb;11(2):330-3 PMID: 803470
  9. Dengue virus-induced modifications of host cell membranes.
    J Virol. 1975 Oct;16(4):1017-26 PMID: 1165590
  10. Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
    Immunochemistry. 1971 Dec;8(12):1175-9 PMID: 5150003
  11. Immunological and biophysical separation of dengue-2 antigens.
    J Virol. 1971 Jan;7(1):15-23 PMID: 5543428
  12. Dengue virions and antigens in brain and serum of infected mice.
    J Virol. 1970 Oct;6(4):500-6 PMID: 5497897
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.13.6.1699-1709.1976
Published
1976-06-00
Pages
1699-709
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC420822
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.13.6.1699-1709.1976 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2