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PMID: 787766 Published · ppublish English Journal Article

Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4.

Molecular & general genetics : MGG ·Vol. 147 ·No. 3 ·1976-09-23 ·Pages 315-23

Jacob AE, Cresswell JM, Hedges RW, Coetzee JN, Beringer JE

Abstract

Plasmids have been constructed by insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4 (an R factor of group P). Such recombinant plasmids retain the wide host range of the parental plasmid, being as efficiently transmissible as the unmodified RP4 and are stably maintained in rapidly growing cultures. The recombinant plasmids, even though each contained a DNA sequence absolutely identical with that of the host strain, are no more efficient at mobilizing the transfer of chromosomal genetic information from that host strain than was unmodified RP4. We therefore conclude that an unknown factor must be essential in the process of chromosome mobilization and rate limiting for that process.

MeSH Terms
Ampicillin/pharmacology DNA, Bacterial DNA, Recombinant Escherichia coli/drug effects Extrachromosomal Inheritance Kanamycin/pharmacology Plasmids Proteus mirabilis R Factors Recombination, Genetic Rhizobium
Chemicals
DNA, Bacterial DNA, Recombinant Kanamycin Ampicillin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jacob A E
Cresswell J M
Hedges R W
Coetzee J N
Beringer J E
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43 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00582883
Published
1976-09-23
Pages
315-23
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00582883
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