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PMID: 787932 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The kinetics of E. coli RNA polymerase.

Nucleic acids research ·Vol. 3 ·No. 9 ·1976-09-00 ·Pages 2207-22

Solage A, Cedar H

Abstract

Using an assay specific for chain elongation of E. coli RNA polymerase the kinetics of this propagation reaction have been studied. The kinetic behaviour is consistent woth the mathematical model formulated for this multisubstrate enzyme. The effect of increasing salt concentration on the kinetics of the reaction indicated that DNA unwinding is probably a necessary step in the propagation step, although this may not be the rate limiting step under all conditions.

MeSH Terms

Adenosine Triphosphate/pharmacology Animals Chickens Cytosine Nucleotides/pharmacology DNA/metabolism DNA, Viral/metabolism Escherichia coli/enzymology Guanosine Triphosphate/pharmacology Kinetics Models, Biological Potassium Chloride/pharmacology RNA, Bacterial/biosynthesis Temperature Uracil Nucleotides/pharmacology

Chemicals

Cytosine Nucleotides DNA, Viral RNA, Bacterial Uracil Nucleotides Potassium Chloride Guanosine Triphosphate Adenosine Triphosphate DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Solage A

Cedar H

References (14)

14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.9.2207

Published

1976-09-00

Pages

2207-22

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343077

Subset

External Links

PubMed source DOI: 10.1093/nar/3.9.2207 PMC Full Text View journal details

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