Arangement of transfer-RNA -genes in yeast.

Nucleic acids research ·Vol. 3 ·No. 9 ·1976-09-00 ·Pages 2379-86

Feldman H

Abstract

The redundancy and the arrangement of the genes for specific transfer ribonucleic acids in yeast were studied by the hybridization techniques developed by Birnstiel et al., e.g.[1]. The redundancy was found to be in the order of 10 genes for tRNA1Met, tRNA3Met, tRNA2Ser, and tRNA-Pro. High molecular weight yeast DNA was fractionated by density gradient centrifugation in cesium chloride and the [32p]tRNAs were hybridized to the single fractions. The results together with earlier findings [2] suggest that the cistrons for these tRNAs are arranged in tandem interspersed by 6 to 10 times longer segments of spacer DNA which varies in (G+C) content for the different tRNA species.

MeSH Terms

DNA Genes Methionine Molecular Weight Nucleic Acid Hybridization Nucleotides/analysis Proline RNA, Transfer Saccharomyces cerevisiae Serine

Chemicals

Nucleotides Serine DNA RNA, Transfer Proline Methionine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Feldman H

References (15)

15 references, click to expand

LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER.
Proc Natl Acad Sci U S A. 1965 Apr;53:737-45 PMID: 14324529
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
J Mol Biol. 1962 Jun;4:430-43 PMID: 14498379
Yeast super-suppressors are altered tRNAs capable of translating a nonsense codon in vitro.
Cell. 1975 Nov;6(3):269-77 PMID: 802681
The primary structure of yeast initiator transfer ribonucleic acid.
Biochem Biophys Res Commun. 1972 Oct 17;49(2):508-15 PMID: 4344891
Kinetic complexity of RNA molecules.
J Mol Biol. 1972 Jan 14;63(1):21-39 PMID: 4622766
Physical linkage of the 5 S cistrons to the 18 S and 28 S ribosomal RNA cistrons in Saccharomyces cerevisiae.
J Mol Biol. 1973 Sep 25;79(3):521-30 PMID: 4585976
Characterization of precursors to tRNA in yeast.
FEBS Lett. 1973 Dec 1;37(2):129-33 PMID: 4587214
Clustering of transfer RNA genes of Xenopus laevis.
J Mol Biol. 1973 Sep 15;79(2):411-29 PMID: 4760136
A two-dimensional fractionation procedure for radioactive nucleotides.
J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels.
FEBS Lett. 1975 Feb 1;50(2):185-9 PMID: 1089563
The primary structure of a non-initiating methionine specific tRNA from brewer's yeast.
FEBS Lett. 1975 Sep 15;57(2):145-8 PMID: 1100431
Purification of tDNA from yeast.
Hoppe Seylers Z Physiol Chem. 1975 Nov;356(11):1703-8 PMID: 1107199
Characteristics of DNA fractionated on benzoylated DEAE-cellulose.
Hoppe Seylers Z Physiol Chem. 1975 Nov;356(11):1693-701 PMID: 1107198
Yeast suppressors of UAA and UAG nonsense codons work efficiently in vitro via tRNA.
Cell. 1976 Mar;7(3):381-90 PMID: 947546
The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae.
J Mol Biol. 1969 Mar 14;40(2):261-77 PMID: 5365012

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.9.2379

Published

1976-09-00

Pages

2379-86

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343092

Subset

External Links

PubMed source DOI: 10.1093/nar/3.9.2379 PMC Full Text View journal details

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