Abstract
Rad51 protein of Saccharomyces cerevisiae is a structural homolog of the Escherichia coli recombination enzyme RecA. In yeast, the Rad51 protein is required for mitotic and meiotic recombination and for repair of double-strand breaks in DNA. We have used antibodies raised against the homologous human protein, HsRad51, expressed in E. coli, to visualize the spatial distribution of the protein in mammalian somatic and meiotic cells. In cultured human cells, the HsRad51 protein is concentrated in multiple discrete foci in the nucleoplasm; it is largely absent from cytoplasm and nucleoli. After treatment of cells with methyl methanesulfonate, ultraviolet irradiation, or 137Cs irradiation, the percentage of cells with HsRad51 protein immunofluorescence increases; the same cells show unscheduled DNA synthesis. Induction of Rad51 foci is blocked by inhibitors of transcription. In mouse pachytene spermatocytes, the mouse homolog of Rad51 protein is highly enriched in synaptonemal complexes that are formed between the paired homologous chromosomes during meiotic prophase. We conclude that the mammalian proteins homologous to yeast Rad51 are involved in repair of DNA damage and recombinational repair during meiosis.
MeSH Terms
Animals
Base Sequence
Cell Line
Cell Nucleus/drug effects,metabolism,radiation effects
Cesium Radioisotopes
Chromosomes/drug effects,physiology,radiation effects
Cloning, Molecular
DNA Damage
DNA Primers
DNA Repair
DNA Replication
DNA-Binding Proteins/biosynthesis,isolation & purification
Dactinomycin/pharmacology
Fibroblasts/cytology,metabolism
Gene Library
Humans
Kidney
Male
Meiosis
Mice
Molecular Sequence Data
Polymerase Chain Reaction
Rad51 Recombinase
Recombinant Fusion Proteins/biosynthesis
Recombination, Genetic/drug effects,radiation effects
Saccharomyces cerevisiae/genetics
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
Spermatocytes/cytology,metabolism
Synaptonemal Complex/drug effects,radiation effects
Thymus Gland/metabolism
Transcription, Genetic/drug effects
Chemicals
Cesium Radioisotopes
DNA Primers
DNA-Binding Proteins
Recombinant Fusion Proteins
Saccharomyces cerevisiae Proteins
Dactinomycin
RAD51 protein, S cerevisiae
RAD51 protein, human
Rad51 Recombinase
Rad51 protein, mouse
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Haaf T
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510.
Golub E I
Reddy G
Radding C M
Ward D C
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