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PMID: 7894488 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 300 kilobase interval genetic map of rice including 883 expressed sequences.

Nature genetics ·Vol. 8 ·No. 4 ·1994-12-00 ·Pages 365-72

Kurata N, Nagamura Y, Yamamoto K, Harushima Y, Sue N, Wu J, Antonio BA, Shomura A, Shimizu T, Lin SY

Abstract

We have constructed a high resolution rice genetic map containing 1,383 DNA markers at an average interval of 300 kilobases (kb). The markers, distributed along 1,575 cM on 12 linkage groups, comprise 883 cDNAs, 265 genomic DNAs, 147 randomly amplified polymorphic DNAs (RAPD) and 88 other DNAs. cDNAs were derived from rice root and callus, analysed by single-run sequencing and searched for similarities with known proteins. Nearly 260 rice genes are newly identified and mapped, and genomic DNA and cloned RAPD fragments were also sequenced to generate STSs. Our map is the first significant gene expression map in plants. It is also the densest genetic map available in plants and the first to be backed up comprehensively by clone sequence data.

MeSH Terms
Chromosome Mapping DNA, Complementary Genes, Plant Genetic Markers Humans Molecular Sequence Data Oryza/genetics
Chemicals
DNA, Complementary Genetic Markers
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Kurata N
Rice Genome Research Program (RGP), National Institute of Agrobiological Resources/Institute of Society for TechnoInnovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
Nagamura Y
Yamamoto K
Harushima Y
Sue N
Wu J
Antonio B A
Shomura A
Shimizu T
Lin S Y
Article Info
Journal
Nature genetics
Abbr.
Nat Genet
ISSN
1061-4036
Published
1994-12-00
Pages
365-72
Language
English
Region
United States
NLM ID
9216904
Subset
IM
Databases
GENBANK
D15075, D15076, D15077, D15078, D15079, D15080, D15081, D15082, D15083, D15084, D15085, D15086, D15087, D15088, D15089, D15090, D15091, D15092, D15093, D15094, D15095, D15096, D15097, D15098, D15099, D15100, D15101, D15102, D15103, D15104
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