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PMID: 78963 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Association of the H-Y male antigen with beta2-microglobulin on human lymphoid and differentiated mouse teratocarcinoma cell lines.

The Journal of experimental medicine ·Vol. 148 ·No. 1 ·1978-07-01 ·Pages 58-70

Fellous M, Günther E, Kemler R, Wiels J, Berger R, Guenet JL, Jakob H, Jacob F

Abstract

The expression of the H-Y antigen has been tested on several human lymphoid lines and mouse teratocarcinoma cell lines during differentiation. The human male lymphoid cell line Raji is a very useful target for studies of the H-Y antigen by lymphocytotoxicity test with rat anti-H-Y sera. With a few exceptions, all cells carrying the Y chromosome were H-Y positive. One of the exceptions is the human Daudi cell line which, besides lacking H-Y antigen, also lacks beta2-microglobulin. We have studied a possible association between the H-Y antigen, beta2-microglobulin, and HLA antigen with redistribution experiments. The results strongly suggest that H-Y antigen is not associated with HLA antigens but with beta2-microglobulin.

MeSH Terms
Animals Beta-Globulins/immunology Cell Line Cell Membrane/immunology Cells, Cultured Cytotoxicity Tests, Immunologic Epitopes Female HLA Antigens Histocompatibility Antigens Humans Immune Sera In Vitro Techniques Lymphocytes/immunology Male Rats Sex Chromosomes Teratoma/immunology Y Chromosome beta 2-Microglobulin/immunology
Chemicals
Beta-Globulins Epitopes HLA Antigens Histocompatibility Antigens Immune Sera beta 2-Microglobulin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Fellous M
Günther E
Kemler R
Wiels J
Berger R
Guenet J L
Jakob H
Jacob F
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.148.1.58
Published
1978-07-01
Pages
58-70
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2184925
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.148.1.58 PMC Full Text View journal details
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