Home LiteratureArticle Details
PMID: 7896711 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A carbon starvation survival gene of Pseudomonas putida is regulated by sigma 54.

Journal of bacteriology ·Vol. 177 ·No. 7 ·1995-04-00 ·Pages 1850-9

Kim Y, Watrud LS, Matin A

Abstract

By using mini-Tn5 transposon mutagenesis, two mutants of Pseudomonas putida ATCC 12633 were isolated which showed a marked increase in their sensitivity to carbon starvation; these mutants are presumably affected in the Pex type of proteins that P. putida induces upon carbon starvation (M. Givskov, L. Eberl, and S. Molin, J. Bacteriol. 176:4816-4824, 1994). The affected genes in our mutants were induced about threefold upon carbon starvation. The promoter region of the starvation gene in the mutant MK107 possessed a strong sigma 54-type-promoter sequence, and deletion analysis suggested that this was the major promoter regulating expression; this was confirmed by transcript mapping in rpoN+ and rpoN mutant backgrounds. The deletion analysis implicated a sequence upstream of the sigma 54 promoter, as well as a region downstream of the transcription start site, in the functioning of the promoter. Two sigma 70-type Pribnow boxes were also detected in the promoter region, but their transcriptional activity in the wild type was very weak. However, in a sigma 54-deficient background, these promoters became stronger. The mechanism and possible physiological role of this phenomenon and the possibility that the sequence upstream of the sigma 54 promoter may have a role in carbon sensing are discussed.

MeSH Terms
Base Sequence Carbon/metabolism Cloning, Molecular DNA Transposable Elements DNA-Binding Proteins DNA-Directed RNA Polymerases Genes, Bacterial Molecular Sequence Data Promoter Regions, Genetic Pseudomonas putida/genetics,metabolism RNA Polymerase Sigma 54 Sigma Factor/genetics,physiology Trichloroethylene/metabolism
Chemicals
DNA Transposable Elements DNA-Binding Proteins Sigma Factor Trichloroethylene Carbon DNA-Directed RNA Polymerases RNA Polymerase Sigma 54
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kim Y
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.
Watrud L S
Matin A
References (45)
45 references, click to expand
  1. Construction and use of a gene bank of Alcaligenes eutrophus in the analysis of ribulose bisphosphate carboxylase genes.
    J Bacteriol. 1984 Sep;159(3):973-8 PMID: 6090401
  2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  3. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector.
    Gene. 1986;48(1):119-31 PMID: 3549457
  4. Separation of temporal control and trans-acting modulation of flagellin and chemotaxis genes in Caulobacter.
    Mol Gen Genet. 1987 Feb;206(2):300-6 PMID: 3473275
  5. Initiation of transcription at the bacterial glnAp2 promoter by purified E. coli components is facilitated by enhancers.
    Cell. 1987 Sep 25;50(7):1039-46 PMID: 3304660
  6. Microbial ecology of the terrestrial subsurface.
    Adv Appl Microbiol. 1988;33:107-72 PMID: 3041739
  7. Differential regulation by cyclic AMP of starvation protein synthesis in Escherichia coli.
    J Bacteriol. 1988 Sep;170(9):3903-9 PMID: 2842291
  8. Starvation-induced cross protection against heat or H2O2 challenge in Escherichia coli.
    J Bacteriol. 1988 Sep;170(9):3910-4 PMID: 3045081
  9. Involvement of Pseudomonas putida RpoN sigma factor in regulation of various metabolic functions.
    J Bacteriol. 1989 Aug;171(8):4326-33 PMID: 2666396
  10. Expression of sigma 54 (ntrA)-dependent genes is probably united by a common mechanism.
    Microbiol Rev. 1989 Sep;53(3):367-76 PMID: 2677638
  11. Genetic basis of starvation survival in nondifferentiating bacteria.
    Annu Rev Microbiol. 1989;43:293-316 PMID: 2478072
  12. In vivo studies on the interaction of RNA polymerase-sigma 54 with the Klebsiella pneumoniae and Rhizobium meliloti nifH promoters. The role of NifA in the formation of an open promoter complex.
    J Mol Biol. 1989 Nov 5;210(1):65-77 PMID: 2685331
  13. The rpoN gene product of Pseudomonas aeruginosa is required for expression of diverse genes, including the flagellin gene.
    J Bacteriol. 1990 Jan;172(1):389-96 PMID: 2152909
  14. Starvation-induced cross protection against osmotic challenge in Escherichia coli.
    J Bacteriol. 1990 May;172(5):2779-81 PMID: 2185233
  15. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria.
    J Bacteriol. 1990 Nov;172(11):6557-67 PMID: 2172216
  16. Responses to multiple-nutrient starvation in marine Vibrio sp. strain CCUG 15956.
    J Bacteriol. 1990 Dec;172(12):7085-97 PMID: 1701428
  17. Role of RpoH, a heat shock regulator protein, in Escherichia coli carbon starvation protein synthesis and survival.
    J Bacteriol. 1991 Mar;173(6):1992-6 PMID: 2002001
  18. Molecular and functional characterization of a carbon starvation gene of Escherichia coli.
    J Mol Biol. 1991 Mar 5;218(1):129-40 PMID: 1848300
  19. The molecular basis of carbon-starvation-induced general resistance in Escherichia coli.
    Mol Microbiol. 1991 Jan;5(1):3-10 PMID: 2014002
  20. Identification of a central regulator of stationary-phase gene expression in Escherichia coli.
    Mol Microbiol. 1991 Jan;5(1):49-59 PMID: 1849609
  21. An upstream XylR- and IHF-induced nucleoprotein complex regulates the sigma 54-dependent Pu promoter of TOL plasmid.
    EMBO J. 1991 May;10(5):1159-67 PMID: 2022186
  22. The putative sigma factor KatF has a central role in development of starvation-mediated general resistance in Escherichia coli.
    J Bacteriol. 1991 Jul;173(13):4188-94 PMID: 2061293
  23. Localization and functional analysis of structural and regulatory dehalogenase genes carried on DEH from Pseudomonas putida PP3.
    J Bacteriol. 1992 Mar;174(6):1941-7 PMID: 1312534
  24. Identification of pilR, which encodes a transcriptional activator of the Pseudomonas aeruginosa pilin gene.
    J Bacteriol. 1992 Jun;174(11):3514-21 PMID: 1317379
  25. Starvation-inducible loci of Salmonella typhimurium: regulation and roles in starvation-survival.
    Mol Microbiol. 1992 Jun;6(11):1467-76 PMID: 1320726
  26. Role of phosphorylated metabolic intermediates in the regulation of glutamine synthetase synthesis in Escherichia coli.
    J Bacteriol. 1992 Oct;174(19):6061-70 PMID: 1356964
  27. Alginate synthesis in Pseudomonas aeruginosa: environmental regulation of the algC promoter.
    J Bacteriol. 1992 Dec;174(23):7680-8 PMID: 1447138
  28. flhF, a Bacillus subtilis flagellar gene that encodes a putative GTP-binding protein.
    Mol Microbiol. 1992 Sep;6(18):2705-13 PMID: 1447978
  29. Survival of hunger and stress: the role of rpoS in early stationary phase gene regulation in E. coli.
    Cell. 1993 Jan 29;72(2):165-8 PMID: 8425216
  30. The putative sigma factor KatF is regulated posttranscriptionally during carbon starvation.
    J Bacteriol. 1993 Apr;175(7):2143-9 PMID: 8458856
  31. KatF (sigma S) synthesis in Escherichia coli is subject to posttranscriptional regulation.
    J Bacteriol. 1993 Apr;175(7):2150-3 PMID: 8458857
  32. Construction and use of a new broad-host-range lacZ transcriptional fusion vector, pHRP309, for gram- bacteria.
    Gene. 1993 Oct 29;133(1):23-30 PMID: 8224891
  33. The stationary phase of the bacterial life cycle.
    Annu Rev Microbiol. 1993;47:855-74 PMID: 8257118
  34. Responses to nutrient starvation in Pseudomonas putida KT2442: analysis of general cross-protection, cell shape, and macromolecular content.
    J Bacteriol. 1994 Jan;176(1):7-14 PMID: 8282712
  35. Transcriptional control of gonococcal pilE expression: involvement of an alternate sigma factor.
    Gene. 1993 Dec 31;137(2):233-6 PMID: 8299953
  36. Regulation of the rpoN, ORF102 and ORF154 genes in Pseudomonas putida.
    FEMS Microbiol Lett. 1994 Jan 15;115(2-3):177-84 PMID: 8138132
  37. Starvation promoters of Escherichia coli. Their function, regulation, and use in bioprocessing and bioremediation.
    Ann N Y Acad Sci. 1994 May 2;721:277-91 PMID: 8010678
  38. Characterization of the sigma 38-dependent expression of a core Escherichia coli starvation gene, pexB.
    J Bacteriol. 1994 Jul;176(13):3928-35 PMID: 8021175
  39. Sensing starvation: a homoserine lactone--dependent signaling pathway in Escherichia coli.
    Science. 1994 Jul 22;265(5171):537-9 PMID: 7545940
  40. Responses to nutrient starvation in Pseudomonas putida KT2442: two-dimensional electrophoretic analysis of starvation- and stress-induced proteins.
    J Bacteriol. 1994 Aug;176(16):4816-24 PMID: 8050994
  41. Integration host factor and sequences downstream of the Pseudomonas aeruginosa algD transcription start site are required for expression.
    J Bacteriol. 1994 Aug;176(16):5068-76 PMID: 8051019
  42. In a class of its own--the RNA polymerase sigma factor sigma 54 (sigma N).
    Mol Microbiol. 1993 Dec;10(5):903-9 PMID: 7934866
  43. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  44. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  45. Starvation proteins in Escherichia coli: kinetics of synthesis and role in starvation survival.
    J Bacteriol. 1986 Nov;168(2):486-93 PMID: 3536847
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.7.1850-1859.1995
Published
1995-04-00
Pages
1850-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC176816
Subset
IM
Databases
GENBANK
S76861
External Links
PubMed source DOI: 10.1128/jb.177.7.1850-1859.1995 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2