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PMID: 7896877 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A novel pool of protein phosphatase 2A is associated with microtubules and is regulated during the cell cycle.

The Journal of cell biology ·Vol. 128 ·No. 6 ·1995-03-00 ·Pages 1131-44

Sontag E, Nunbhakdi-Craig V, Bloom GS, Mumby MC

Abstract

Immunofluorescence microscopy revealed the presence of protein phosphatase 2A (PP2A) on microtubules in neuronal and nonneuronal cells. Interphase and mitotic spindle microtubules, as well as centrosomes, were all labeled with antibodies against individual PP2A subunits, showing that the AB alpha C holoenzyme is associated with microtubules. Biochemical analysis showed that PP2A could be reversibly bound to microtubules in vitro and that approximately 75% of the PP2A in cytosolic extracts could interact with microtubules. The activity of microtubule-associated PP2A was differentially regulated during the cell cycle. Enzymatic activity was high during S phase and intermediate during G1, while the activity in G2 and M was 20-fold lower than during S phase. The amount of microtubule-bound PP2A remained constant throughout the cell cycle, implying that cell cycle regulation of its enzymatic activity involves factors other than microtubules. These results raise the possibility that PP2A regulates cell cycle-dependent microtubule functions, such as karyokinesis and membrane transport.

MeSH Terms
Animals Cattle Cell Cycle Cells, Cultured Fluorescent Antibody Technique Haplorhini Mice Microtubules/metabolism Phosphoprotein Phosphatases/metabolism Protein Binding Protein Phosphatase 2 Rats
Chemicals
Phosphoprotein Phosphatases Protein Phosphatase 2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sontag E
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9041.
Nunbhakdi-Craig V
Bloom G S
Mumby M C
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.128.6.1131
Published
1995-03-00
Pages
1131-44
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120410
Subset
IM
Grants
NIGMS NIH HHS · GM49505 · United States
NHLBI NIH HHS · HL31107 · United States
NINDS NIH HHS · NS23868 · United States
External Links
PubMed source DOI: 10.1083/jcb.128.6.1131 PMC Full Text View journal details
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