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PMID: 790160 Published · ppublish English Journal Article

Regulation of the level of yeasts citrate synthase by oxygen availability.

Molecular and cellular biochemistry ·Vol. 12 ·No. 3 ·1976-09-30 ·Pages 161-9

Nuñez de castro I, Arias de Saavedra JM, Machado A, Mayor F

Abstract

The activity of yeasts citrate synthase in cells grown under different hypoxic conditions has been investigated. A linear relationship between the citrate synthase activity and the respiratory capacity of the cells has been found. When Saccharomyces cerevisiae was grown on fermentable substrates the activity decreased as the concentration of sugars in the medium increased. The enzyme of the yeast Rhodoturula showed a high activity in spite of the existence of high sugar concentration in the culture medium. Neither feed-back repression by glutamate nor feed-forward induction by ammonia has been found in bakers' yeast. The results suggest that the regulation of the enzyme by oxygen availability takes place by the ""de novo'' synthesis of the enzyme.

MeSH Terms
Acetates/pharmacology Aerobiosis Citrate (si)-Synthase/metabolism Cycloheximide/pharmacology Ethanol/pharmacology Gluconates/pharmacology Glycerol/pharmacology Hexoses/metabolism,pharmacology Kinetics Oxo-Acid-Lyases/metabolism Oxygen Consumption Saccharomyces cerevisiae/drug effects,enzymology Species Specificity
Chemicals
Acetates Gluconates Hexoses Ethanol Cycloheximide Citrate (si)-Synthase Oxo-Acid-Lyases Glycerol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nuñez de castro I
Arias de Saavedra J M
Machado A
Mayor F
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21 references, click to expand
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Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF01741714
Published
1976-09-30
Pages
161-9
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01741714 View journal details
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