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PMID: 7907418 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A proline-rich transcriptional activation domain in murine HOXD-4 (HOX-4.2).

Nucleic acids research ·Vol. 22 ·No. 3 ·1994-02-11 ·Pages 376-82

Rambaldi I, Kovàcs EN, Featherstone MS

Abstract

The product of the murine Hoxd-4 (Hox-4.2) gene is a transcription factor that acts upon an autoregulatory element in Hoxd-4 upstream sequences (1). Using this activity as an assay in transient transfections of P19 embryonal carcinoma (EC) cells, we performed a mutational analysis to map functional domains in the HOXD-4 protein. The importance of the homeodomain was shown by a single amino acid change in this region that abolished activity. Deletion analysis revealed that many evolutionarily conserved regions outside of the homeodomain were dispensable for activation in our assay. Fusions to the GAL4 DNA-binding domain mapped a transcriptional activation function to the HOXD-4 proline-rich N-terminus. The proline-rich transcription factor AP2 squelched activation by HOXD-4 and by GAL4/HOXD-4 N-terminus fusion proteins. Together, these results suggest that HOXD-4 harbors a transcriptional activation domain of the proline-rich type.

Related Genes
Hoxd-4
MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA-Binding Proteins/chemistry,genetics,metabolism Gene Expression Regulation Genes, Homeobox Mice Molecular Sequence Data Mutagenesis, Site-Directed Proline Promoter Regions, Genetic Recombinant Fusion Proteins/chemistry Structure-Activity Relationship Transcription Factor AP-2 Transcription Factors/chemistry,genetics,metabolism Transcription, Genetic Transcriptional Activation
Chemicals
DNA-Binding Proteins Hoxd4 protein, mouse Recombinant Fusion Proteins Transcription Factor AP-2 Transcription Factors Proline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rambaldi I
McGill Cancer Centre, Montréal, Québec, Canada.
Kovàcs E N
Featherstone M S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.3.376
Published
1994-02-11
Pages
376-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC523592
Subset
IM
Databases
GENBANK
J03770
External Links
PubMed source DOI: 10.1093/nar/22.3.376 PMC Full Text View journal details
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