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PMID: 7908060 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The influenza virus NS1 protein is a poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A).

Journal of virology ·Vol. 68 ·No. 4 ·1994-04-00 ·Pages 2425-32

Qiu Y, Krug RM

Abstract

The influenza virus NS1 protein inhibits the nuclear export of a spliced viral mRNA, NS2 mRNA (F. V. Alonso-Caplen, M. E. Nemeroff, Y. Qiu, and R. M. Krug, Genes Dev. 6:255-267, 1992). To identify the sequence in NS2 mRNA that is recognized by the NS1 protein, we developed a gel shift assay for the formation of specific RNA-protein complexes. With this assay, it was established that the NS1 protein binds to the poly(A) sequence at the 3' end of NS2 mRNA and of other mRNAs. In addition, the NS1 protein was shown to bind to poly(A) itself. This specificity was also observed in vivo. The NS1 protein inhibited the nuclear export of every poly(A)-containing mRNA that was tested. In contrast, the NS1 protein failed to inhibit the nuclear export of an mRNA whose 3' end was generated by cleavage without subsequent addition of poly(A). Addition of poly(A) to this mRNA enabled the NS1 protein to inhibit mRNA export. The implications of these results for the role of the NS1 protein during virus infection are discussed.

MeSH Terms
Binding, Competitive Biological Transport Cell Nucleus/metabolism Models, Biological Orthomyxoviridae/metabolism Poly A/metabolism Protein Binding RNA, Messenger/metabolism RNA-Binding Proteins/metabolism Ribonucleoproteins/metabolism Viral Nonstructural Proteins/metabolism
Chemicals
INS1 protein, influenza virus RNA, Messenger RNA-Binding Proteins Ribonucleoproteins Viral Nonstructural Proteins Poly A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Qiu Y
Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08855-1179.
Krug R M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.4.2425-2432.1994
Published
1994-04-00
Pages
2425-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236720
Subset
IM
Grants
NIAID NIH HHS · AI 11772 · United States
External Links
PubMed source DOI: 10.1128/JVI.68.4.2425-2432.1994 PMC Full Text View journal details
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