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PMID: 7909609 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification and physiological evaluation of a guanylate cyclase activating protein from retinal rods.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 9 ·1994-04-26 ·Pages 4014-8

Gorczyca WA, Gray-Keller MP, Detwiler PB, Palczewski K

Abstract

In retinal rods light triggers a cascade of enzymatic reactions that increases cGMP hydrolysis and generates an electrical signal by causing closure of cGMP-gated ion channels in the photoreceptor outer segment. This leads to a decrease in internal Ca, which activates guanylate cyclase and promotes photoresponse recovery by stimulating the resynthesis of cGMP. We report here that the activation of guanylate cyclase by low Ca is mediated by an approximately 20-kDa protein purified from bovine rod outer segments by using DEAE-Sepharose, hydroxylapatite, and reverse-phase chromatographies. In a reconstituted system, this protein restores the Ca-sensitive regulation of guanylate cyclase and when dialyzed into functionally intact lizard rod outer segment decreases the sensitivity, time to peak, and recovery time of the flash response.

MeSH Terms
Animals Calcium/physiology Cattle Enzyme Activation Eye Proteins/isolation & purification,physiology Guanylate Cyclase/metabolism Molecular Weight Rod Cell Outer Segment/enzymology Vision, Ocular/physiology
Chemicals
Eye Proteins Guanylate Cyclase Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gorczyca W A
Department of Ophthalmology, School of Medicine, University of Washington, Seattle 98195.
Gray-Keller M P
Detwiler P B
Palczewski K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.9.4014
Published
1994-04-26
Pages
4014-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43713
Subset
IM
Grants
NEI NIH HHS · EY 02048 · United States
NEI NIH HHS · EY 08061 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.9.4014 PMC Full Text View journal details
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