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PMID: 7919010 Published · ppublish English Journal Article

Simultaneous measurement of ciliary beating and intracellular calcium.

Biophysical journal ·Vol. 67 ·No. 1 ·1994-07-00 ·Pages 377-80

Korngreen A, Priel Z

Abstract

A novel system for measuring, simultaneously, ciliary beating and intracellular free calcium is presented. The advantages and dynamic nature of the system are demonstrated by measuring the effects of the calcium ionophore lonomycin and of extracellular ATP on ciliated rabbit trachea. The results are discussed with regard to the ciliary and calcium stimulation.

MeSH Terms
Adenosine Triphosphate/pharmacology Animals Calcium/metabolism Cilia/drug effects,physiology Fluorescent Dyes Fura-2/analogs & derivatives Ionomycin/pharmacology Kinetics Muscle, Smooth/drug effects,physiology Organ Culture Techniques Rabbits Time Factors Trachea/physiology
Chemicals
Fluorescent Dyes fura-2-am Ionomycin Adenosine Triphosphate Calcium Fura-2
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Korngreen A
Department of Chemistry, Ben-Gurion University of Negev, Beer-Sheva, Israel.
Priel Z
References (18)
18 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(94)80492-8
Published
1994-07-00
Pages
377-80
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1225369
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(94)80492-8 PMC Full Text View journal details
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