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PMID: 791903 Published · ppublish English Journal Article

Histochemical lectin affinity technique by means of FITC-labeled serum protein fractions.

Histochemistry ·Vol. 49 ·No. 1 ·1976-10-07 ·Pages 67-72

Thoss K, Roth J

Abstract

A two-step affinity technique is described for light microscopic demonstration of the Concanavalin A, Agaricus bisporus lectin and Ricinus communis lectin binding sites by means of various FITC-labeled human and rabbit serum protein fractions. Experiments for the visualization of the Lens culinaris lectin and the Pisum sativum lectin binding sites gaves negative results. The technique consist of two reaction steps which involve the incubation of tissue sections in the lectins followed by the visualization of receptor-bound lectins with FITC-labeled serum protein fractions basing on their carbohydrate content. The specificity of the technique could be demonstrated by the addition of the hapten or by incubation in the FITC-labeled serum protein fractions only. In contrast to the direct or indirect staining methods only very small amounts of purified lectins are necessary.

MeSH Terms
Animals Fluorescent Antibody Technique Lectins Rats Receptors, Concanavalin A/analysis Receptors, Drug/analysis
Chemicals
Lectins Receptors, Concanavalin A Receptors, Drug
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thoss K
Roth J
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20 references, click to expand
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00490127
Published
1976-10-07
Pages
67-72
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00490127
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