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PMID: 791937 Published · ppublish English Journal Article

Detection, with the dye phloxine B, of yeast mutants unable to utilize nitrogenous substances as the sole nitrogen source.

Journal of bacteriology ·Vol. 128 ·No. 3 ·1976-12-00 ·Pages 851-2

Middelhoven WJ, Broekhuizen B, van Eijk J

Abstract

Yeast mutants unable to degrade certain nitrogen compounds produce characteristic small red colonies on an agar medium containing the red dye phloxine B, galactose, the test nitrogen compound, and a small amount of ammonium chloride.

MeSH Terms
Arginine/metabolism Fluoresceins Genes Methods Mutation Saccharomyces cerevisiae/metabolism
Chemicals
Fluoresceins Arginine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Middelhoven W J
Broekhuizen B
van Eijk J
References (7)
7 references, click to expand
  1. Factors Affecting the Survival of Auxotrophs and Prototrophs of Saccharomyces cerevisiae in Mixed Populations.
    J Bacteriol. 1966 Aug;92(2):297-301 PMID: 16562110
  2. An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'.
    Nature. 1966 Jul 9;211(5045):206-7 PMID: 5965527
  3. DIAGNOSTIC COLOR DIFFERENTIATION PLATES FOR HEREDITARY RESPIRATION DEFICIENCY IN YEAST.
    J Bacteriol. 1963 Aug;86:299-302 PMID: 14058956
  4. The control of ornithinetranscarbamylase activity by arginase in Saccharomyces cerevisiae.
    FEBS Lett. 1969 Apr;3(1):47-49 PMID: 11946965
  5. Use of Magdala red for the detection of auxotrophic mutants of Saccharomyces cerevisiae.
    J Bacteriol. 1966 Mar;91(3):1388 PMID: 5929766
  6. [Optimization of the selection of mutants of Saccharomyces cerevisiae by nystatin].
    Ann Inst Pasteur (Paris). 1971 May;120(5):617-25 PMID: 5088166
  7. Induction and repression of arginase and ornithine transaminase in baker's yeast.
    Antonie Van Leeuwenhoek. 1970;36(1):1-19 PMID: 4912187
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.128.3.851-852.1976
Published
1976-12-00
Pages
851-2
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC232777
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.128.3.851-852.1976 PMC Full Text View journal details
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