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PMID: 7920635 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A map of the human immunoglobulin VH locus completed by analysis of the telomeric region of chromosome 14q.

Nature genetics ·Vol. 7 ·No. 2 ·1994-06-00 ·Pages 162-8

Cook GP, Tomlinson IM, Walter G, Riethman H, Carter NP, Buluwela L, Winter G, Rabbitts TH

Abstract

Analysis of the telomeric region of chromosome 14q has enabled us to complete a map of the immunoglobulin VH locus which accounts for almost all VH segments known to rearrange in B-lymphocytes. The human germline VH repertoire consists of approximately 50 functional VH segments--the exact number depending on the haplotype--spanning 1,100 kilobases upstream of the JH segments. A yeast artificial chromosome used to map these segments was isolated by its ability to provide telomere activity in yeast, suggesting that the VH locus may be located within a few kilobases of the 14q telomere. The limited structural diversity encoded by the functional VH segments demonstrates the importance of combinatorial diversity produced by VDJ joining and the association of heavy and light chains in producing the human antibody repertoire.

MeSH Terms

Amino Acid Sequence Antibody Diversity Base Sequence Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Human, Pair 14 Chromosomes, Human, Pair 8 DNA/genetics Gene Rearrangement, B-Lymphocyte, Heavy Chain Genes, Immunoglobulin Haplotypes Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Molecular Sequence Data Sequence Homology, Amino Acid Translocation, Genetic Tumor Cells, Cultured

Chemicals

Immunoglobulin Heavy Chains Immunoglobulin Variable Region DNA

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Cook G P

MRC Laboratory of Molecular Biology, MRC Centre for Protein Engineering, Cambridge, UK.

Tomlinson I M

Walter G

Riethman H

Carter N P

Buluwela L

Winter G

Rabbitts T H

Article Info

Journal

Nature genetics

Abbr.

Nat Genet

ISSN

1061-4036

DOI

10.1038/ng0694-162

Published

1994-06-00

Pages

162-8

Language

English

Region

United States

NLM ID

9216904

Subset

Databases

GENBANK

Z27502, Z27503, Z27504, Z27505, Z27506, Z27507, Z27508, Z27509, Z27510, Z29977, Z29978, Z29979, Z29980, Z29981, Z29982, Z29983, Z29984, Z29985, Z29986, Z30082, Z30083, Z30095, Z30096

External Links

PubMed source DOI: 10.1038/ng0694-162 View journal details

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A map of the human immunoglobulin VH locus completed by analysis of the telomeric region of chromosome 14q.

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