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PMID: 7923993 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The role of extracellular matrix in human astrocytoma migration and proliferation studied in a microliter scale assay.

Clinical & experimental metastasis ·Vol. 12 ·No. 6 ·1994-11-00 ·Pages 405-15

Berens ME, Rief MD, Loo MA, Giese A

Abstract

Ligands in the extracellular matrix (ECM) are known to mediate migration of normal as well as tumor cells via adhesion molecules such as the integrin receptor family. We develop a microliter scale (15-20 microliters total volume) monolayer migration assay to investigate the ability of astrocytoma cells to disperse on surfaces coated with purified human ECM protein ligands. In this system the rate of radial migration of the cell population was constant over time. For human astrocytoma cell lines U-251 and SF-767, laminin and collagen type IV supported a migratory phenotype; fibronectin and vitronectin only minimally supported migration. The different ECM proteins also influenced growth rate: cells on laminin and collagen had a protracted lag phase. Furthermore, migrating cells seeded on laminin or collagen showed a lower labeling index than did stationary cells in the central, crowded region on the same substrate. This micro-scale migration assay should enable detailed molecular and biochemical studies of the determinants of migration.

MeSH Terms
Astrocytoma/pathology Cell Adhesion Cell Division Cell Movement Extracellular Matrix/physiology Extracellular Matrix Proteins/metabolism Humans In Vitro Techniques Tumor Cells, Cultured
Chemicals
Extracellular Matrix Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Berens M E
Neuro-Oncology Laboratory, Barrow Neurological Institute, Saint Joseph's Hospital and Medical Center, Phoenix, AZ 85013-4496.
Rief M D
Loo M A
Giese A
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Article Info
Journal
Clinical & experimental metastasis
Abbr.
Clin Exp Metastasis
ISSN
0262-0898
DOI
10.1007/BF01755884
Published
1994-11-00
Pages
405-15
Language
English
Region
Netherlands
NLM ID
8409970
Subset
IM
Grants
NINDS NIH HHS · NS27030 · United States
External Links
PubMed source DOI: 10.1007/BF01755884 View journal details
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