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PMID: 792816 Published · ppublish English Journal Article

Specific replacement of Q base in the anticodon of tRNA by guanine catalyzed by a cell-free extract of rabbit reticulocytes.

Nucleic acids research ·Vol. 3 ·No. 10 ·1976-10-00 ·Pages 2593-603

Okada N, Harada F, Nishimura S

Abstract

Guanylation of tRNA by a lysate of rabbit reticulocytes was reported previously by Farkas and Singh. This reaction was investigated further using 18 purified E. coli tRNAs as acceptors. Results showed that only tRNATyr, tRNAHis, tRNAAsn and tRNAAsp which contain the modified nucleoside Q in the anticodon acted as acceptors. Analysis of the nucleotide sequences in the guanylated tRNA showed that guanine specifically replaced Q base in these tRNAs.

MeSH Terms
Animals Anticodon/metabolism Asparagine Base Sequence Escherichia coli Guanine/metabolism Histidine RNA, Bacterial/metabolism RNA, Transfer/metabolism Rabbits Reticulocytes/metabolism Ribonucleosides/metabolism Saccharomyces cerevisiae
Chemicals
Anticodon RNA, Bacterial Ribonucleosides Histidine Guanine Asparagine RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Okada N
Harada F
Nishimura S
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.10.2593
Published
1976-10-00
Pages
2593-603
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343115
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.10.2593 PMC Full Text View journal details
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