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PMID: 7929593 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The alpha v beta 6 integrin promotes proliferation of colon carcinoma cells through a unique region of the beta 6 cytoplasmic domain.

The Journal of cell biology ·Vol. 127 ·No. 2 ·1994-10-00 ·Pages 547-56

Agrez M, Chen A, Cone RI, Pytela R, Sheppard D

Abstract

Cell-matrix interactions are assumed to be important in regulating differentiation and tumor cell growth; however, the precise roles of individual matrix receptors in producing cellular responses are still unclear. We have previously described the alpha v beta 6 integrin, an epithelial cell fibronectin receptor expressed in many carcinoma cell lines. Here we show that heterologous expression of alpha v beta 6 in a human colon carcinoma cell line (SW480) enhances the proliferative capacity of these cells, both in vitro and in vivo in nude mice. This property of alpha v beta 6 correlates with the presence of an 11-amino acid region at the COOH terminus of the beta 6 cytoplasmic domain. This 11-amino acid sequence is required for the growth stimulatory effect, but not for other functions of the beta 6 cytoplasmic domain, such as promoting cell adhesion and focal contact localization.

MeSH Terms
Amino Acid Sequence Animals Antigens, Neoplasm Cell Adhesion Cell Division Cell Line Collagen Colonic Neoplasms/pathology Fibronectins Humans Integrins/chemistry,genetics,physiology Mice Mice, Nude Molecular Sequence Data Receptors, Fibronectin/chemistry,genetics,physiology Transfection Tumor Cells, Cultured
Chemicals
Antigens, Neoplasm Fibronectins Integrins Receptors, Fibronectin integrin alphavbeta6 Collagen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Agrez M
Department of Medicine, University of California, San Francisco.
Chen A
Cone R I
Pytela R
Sheppard D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.127.2.547
Published
1994-10-00
Pages
547-56
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120218
Subset
IM
Grants
NHLBI NIH HHS · HL/A133259 · United States
NHLBI NIH HHS · HL25816 · United States
NHLBI NIH HHS · HL47412 · United States
External Links
PubMed source DOI: 10.1083/jcb.127.2.547 PMC Full Text View journal details
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