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PMID: 7933140 Published · ppublish English Journal Article

Measles virus fusion: role of the cysteine-rich region of the fusion glycoprotein.

Journal of virology ·Vol. 68 ·No. 11 ·1994-11-00 ·Pages 7546-8

Wild TF, Fayolle J, Beauverger P, Buckland R

Abstract

Measles virus (MV) fusion requires the participation of both the fusion (F) and hemagglutinin (H) glycoproteins. The canine distemper virus fusion protein (CDVF) cannot substitute for the measles virus fusion protein (MVF) in this process. Introduction of restriction enzyme sites into the cDNAs of CDVF and MVF by site-directed mutagenesis facilitated the production of chimeric F proteins which were tested for their capacity to give fusion when coexpressed with MVH. Fusion resulted when the amino-terminal half of the MVF cysteine-rich region was transferred to CDVF.

MeSH Terms
Amino Acid Sequence Cysteine/analysis Distemper Virus, Canine/physiology Glycoproteins/chemistry,physiology HeLa Cells Humans Measles virus/physiology Molecular Sequence Data Mutagenesis, Site-Directed Structure-Activity Relationship Viral Fusion Proteins/physiology
Chemicals
Glycoproteins Viral Fusion Proteins Cysteine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wild T F
Institut National de la Santé et de la Recherche Médicale U.404, Institut Pasteur de Lyon, France.
Fayolle J
Beauverger P
Buckland R
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.11.7546-7548.1994
Published
1994-11-00
Pages
7546-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237199
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.11.7546-7548.1994 PMC Full Text View journal details
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