Yeast cell-cycle mutant cdc21 is a temperature-sensitive thymidylate auxotroph.

Molecular & general genetics : MGG ·Vol. 146 ·No. 3 ·1976-08-02 ·Pages 313-5

Game JC

Abstract

Genetic tests with the yeast cell-cycle mutant cdc21 isolated by Hartwell indicate that the CDC21 gene in yeast is the same as the TMP1 gene, whose mutant alleles confer an auxotrophic requirement for thymidine-5'-monophosphate (dTMP). Yeast strains carrying cdc21 can grow at 37 degrees in the presence of dTMP provided that they are premeable to this compound. The gene is shown to be linked to ade2 on Chr. XV, and a case of intragenic complementation between cdc21 and another tmp1 allele is reported.

MeSH Terms

Cell Division Genes Genetic Complementation Test Genetic Linkage Hot Temperature Mutation Saccharomyces cerevisiae/metabolism Thymine Nucleotides/metabolism

Chemicals

Thymine Nucleotides

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Game J C

References (11)

11 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00701257

Published

1976-08-02

Pages

313-5

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00701257

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Yeast cell-cycle mutant cdc21 is a temperature-sensitive thymidylate auxotroph.

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