Kinetic studies on the cleavage of adenovirus DNA by restriction endonuclease Eco RI.

Nucleic acids research ·Vol. 3 ·No. 12 ·1976-12-00 ·Pages 3255-69

Forsblom S, Rigler R, Ehrenberg M, Philipson L

Abstract

The kinetics of cleavage of DNA from Adenovirus Type 1 (Ad1), Type 5 (Ad5) and Type 6 (Ad6) by restriction endonuclease EcoRI was investigated by quantitative evaluation of the fluorescence from ethidium stained DNA fragments separated on agarose gels. The apparent rate constants of cleavage at different cleavage sites have been determined and large differences in the cleavage rates of the individual sites within one type of DNA were found. From the kinetics of cleavage information on the sequence of the DNA fragments can be obtained. The order of the fragment A, B, C, D of Ad6 DNA obtained after complete cleavage by restriction endonuclease Eco RI was found to be A-D-C-B; the order of the corresponding fragments A, B, C of Ad1 and Ad5 DNA was found to be A-C-B.

MeSH Terms

Adenoviruses, Human Base Sequence DNA Restriction Enzymes/metabolism DNA, Viral/analysis,metabolism Endonucleases/metabolism Escherichia coli/enzymology Methods Molecular Weight Structure-Activity Relationship

Chemicals

DNA, Viral Endonucleases DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Forsblom S

Rigler R

Ehrenberg M

Philipson L

References (12)

12 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.12.3255

Published

1976-12-00

Pages

3255-69

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343172

Subset

External Links

PubMed source DOI: 10.1093/nar/3.12.3255 PMC Full Text View journal details

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Kinetic studies on the cleavage of adenovirus DNA by restriction endonuclease Eco RI.

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