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PMID: 7957069 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Voltage-dependent facilitation of a neuronal alpha 1C L-type calcium channel.

The EMBO journal ·Vol. 13 ·No. 21 ·1994-11-01 ·Pages 5032-9

Bourinet E, Charnet P, Tomlinson WJ, Stea A, Snutch TP, Nargeot J

Abstract

Calcium entry into excitable cells through voltage-gated calcium channels can be influenced by both the rate and pattern of action potentials. We report here that a cloned neuronal alpha 1C L-type calcium channel can be facilitated by positive pre-depolarization. Both calcium and barium were effective as charge carriers in eliciting voltage-dependent facilitation. The induction of facilitation was shown to be independent of intracellular calcium levels, G-protein interaction and the level of phosphatase activity. Facilitation was reduced by the injection of inhibitors of protein kinase A and required the coexpression of a calcium channel beta subunit. In contrast, three neuronal non-L-type calcium channels, alpha 1A, alpha 1B and alpha 1E, were not subject to voltage-dependent facilitation when coexpressed with a beta subunit. The results indicate that the mechanism of neuronal L-type calcium channel facilitation involves the interaction of alpha 1 and beta subunits and is dependent on protein kinase A activity. The selective voltage-dependent modulation of L-type calcium channels is likely to play an important role in neuronal physiology and plasticity.

MeSH Terms
Animals Brain Chemistry Calcium/metabolism Calcium Channels/classification,genetics,physiology Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors,metabolism Ion Channel Gating/physiology Membrane Potentials/physiology Neurons/chemistry Oocytes Protein Conformation Rats Recombinant Proteins/metabolism Xenopus
Chemicals
Calcium Channels Recombinant Proteins Cyclic AMP-Dependent Protein Kinases Calcium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bourinet E
Biotechnology Laboratory, University of British Columbia, Vancouver, Canada.
Charnet P
Tomlinson W J
Stea A
Snutch T P
Nargeot J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-11-01
Pages
5032-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395449
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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