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PMID: 7960152 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Immune responsiveness and lymphokine production in patients with tuberculosis and healthy controls.

Infection and immunity ·Vol. 62 ·No. 12 ·1994-12-00 ·Pages 5673-8

Sánchez FO, Rodríguez JI, Agudelo G, García LF

Abstract

The aim of the present study was to determine the profile of immune responsiveness that differentiates patients with tuberculosis (TB) from healthy tuberculin-positive controls. Forty-five patients with pulmonary TB and 16 healthy tuberculin-positive controls, all human immunodeficiency virus negative, were studied. Patients had decreased reactivity to tuberculin, diminished proliferative response to purified protein derivative (PPD), lower concentrations of interleukin-2 (IL-2) and gamma interferon in PPD-stimulated cultures, no increase in the percentage of gamma/delta cells in PPD-stimulated cultures, and higher immunoglobulin G antimycobacterial antibodies compared with control subjects. Furthermore, controls exhibited decreased production of IL-4 by PPD-stimulated cells. Multivariate discriminant and factor analyses demonstrated divergent patterns of immune reactivity against mycobacterial antigens. The association of IL-4 and immunoglobulin G antibody levels in patients, in contrast to the high reactivity to tuberculin, increased proliferation to PPD, and higher levels of IL-2 and gamma interferon observed in healthy controls suggested that most TB patients exhibit a TH2 pattern of immune responsiveness while tuberculin-positive healthy individuals have a TH1 pattern.

MeSH Terms
Adolescent Adult Aged Female Humans Hypersensitivity, Delayed/classification,etiology,immunology Interferon-gamma/biosynthesis Interleukin-2/biosynthesis Interleukin-4/biosynthesis Lymphokines/biosynthesis Male Middle Aged Multivariate Analysis Receptors, Antigen, T-Cell, gamma-delta/immunology T-Lymphocyte Subsets Tuberculin Test Tuberculosis, Pulmonary/classification,etiology,immunology
Chemicals
Interleukin-2 Lymphokines Receptors, Antigen, T-Cell, gamma-delta Interleukin-4 Interferon-gamma
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sánchez F O
Laboratorio Central de Investigaciones, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
Rodríguez J I
Agudelo G
García L F
References (44)
44 references, click to expand
  1. Humoral and cellular immunity in the anergic tuberculosis patient. A prospective study.
    J Allergy Clin Immunol. 1974 Jan;53(1):20-6 PMID: 4202828
  2. Stimulation of human gamma delta T cells by nonpeptidic mycobacterial ligands.
    Science. 1994 Apr 8;264(5156):267-70 PMID: 8146660
  3. Spectrum of immune response abnormalities in different clinical forms of tuberculosis.
    Am Rev Respir Dis. 1977 Feb;115(2):207-12 PMID: 300226
  4. The spectrum of human tuberculosis.
    Clin Exp Immunol. 1977 Feb;27(2):230-7 PMID: 849655
  5. Study of the immunological status of patients with pulmonary tuberculosis.
    Scand J Immunol. 1978;8(4):333-8 PMID: 309628
  6. Cell-mediated immunity in anergic patients with pulmonary tuberculosis.
    Am Rev Respir Dis. 1978 Nov;118(5):827-34 PMID: 736354
  7. Anergy in active pulmonary tuberculosis. A comparison between positive and negative reactors and an evaluation of 5 TU and 250 TU skin test doses.
    Chest. 1980 Jan;77(1):32-7 PMID: 7351142
  8. The immune spectrum in patients with pulmonary tuberculosis.
    Am Rev Respir Dis. 1981 May;123(5):556-9 PMID: 6786150
  9. Enzyme-linked immunosorbent assay (ELISA) for antibodies to purified protein derivative of tuberculin (PPD). IgM-, IgA- and IgG- anti-PPD antibodies in active pulmonary tuberculosis.
    Eur J Respir Dis. 1982 May;63(3):257-62 PMID: 7095027
  10. Nonspecific recruitment of lymphocytes in purified protein derivative-induced lymphocyte proliferative response of patients with tuberculosis.
    Infect Immun. 1982 Aug;37(2):702-9 PMID: 6180987
  11. Detection of immunoglobulin G antibody to purified protein derivative in patients with tuberculosis by radioimmunoassay and enzyme-linked immunosorbent assay.
    J Clin Microbiol. 1982 Jan;15(1):93-6 PMID: 6985299
  12. Formation of total and stable E-rosettes by lymphocytes from patients with pulmonary tuberculosis stimulated by Con A or PPD.
    J Clin Lab Immunol. 1985 Jan;16(1):31-6 PMID: 3981616
  13. T4 lymphopenia in patients with active pulmonary tuberculosis.
    Clin Exp Immunol. 1985 Apr;60(1):49-54 PMID: 3874015
  14. Defective interleukin 2 production and responsiveness in human pulmonary tuberculosis.
    J Exp Med. 1986 May 1;163(5):1162-72 PMID: 2939169
  15. Defective gamma-interferon production in peripheral blood leukocytes of patients with acute tuberculosis.
    J Clin Immunol. 1986 Mar;6(2):146-51 PMID: 3086365
  16. Antigen responsiveness during tuberculosis: regulatory interactions of T cell subpopulations and adherent cells.
    J Lab Clin Med. 1987 Jul;110(1):31-40 PMID: 2439622
  17. IgG antibody level to mycobacterial glycoprotein in pulmonary tuberculosis by ELISA.
    Eur J Respir Dis. 1987 Jul;71(1):37-41 PMID: 3115808
  18. Specific lymphoproliferation, gamma interferon production, and serum immunoglobulin G directed against a purified 32 kDa mycobacterial protein antigen (P32) in patients with active tuberculosis.
    Scand J Immunol. 1988 Feb;27(2):187-94 PMID: 3124263
  19. Immune status in tuberculosis and response to treatment.
    Tubercle. 1988 Jun;69(2):81-94 PMID: 3188236
  20. Immunity against intracellular bacteria: biological effector functions and antigen specificity of T lymphocytes.
    Curr Top Microbiol Immunol. 1988;138:141-76 PMID: 3058388
  21. Stimulation of a major subset of lymphocytes expressing T cell receptor gamma delta by an antigen derived from Mycobacterium tuberculosis.
    Cell. 1989 May 19;57(4):667-74 PMID: 2524273
  22. Is macrophage death on the field of battle essential to victory, or a tactical weakness in immunity against tuberculosis?
    Clin Exp Immunol. 1990 Jun;80(3):301-3 PMID: 2197045
  23. Natural killer cell activity in patients with pulmonary tuberculosis and in healthy controls.
    Tubercle. 1990 Jun;71(2):95-102 PMID: 2120829
  24. Functional diversity of T lymphocytes due to secretion of different cytokine patterns.
    FASEB J. 1991 Feb;5(2):171-7 PMID: 1825981
  25. T-cell proliferative response to antigens secreted by Mycobacterium tuberculosis.
    Infect Immun. 1991 Apr;59(4):1558-63 PMID: 1900811
  26. Murine Th1 and Th2 clones proliferate optimally in response to distinct antigen-presenting cell populations.
    J Immunol. 1991 Mar 15;146(6):1750-8 PMID: 1826010
  27. Selection by two powerful antigens may account for the presence of the major population of human peripheral gamma/delta T cells.
    J Exp Med. 1991 Jun 1;173(6):1311-22 PMID: 1827824
  28. Purified protein derivative of Mycobacterium tuberculosis and excretory-secretory antigen(s) of Toxocara canis expand in vitro human T cells with stable and opposite (type 1 T helper or type 2 T helper) profile of cytokine production.
    J Clin Invest. 1991 Jul;88(1):346-50 PMID: 1829097
  29. Selection of a human T helper type 1-like T cell subset by mycobacteria.
    J Exp Med. 1991 Sep 1;174(3):583-92 PMID: 1831489
  30. Human TH1 and TH2 subsets: doubt no more.
    Immunol Today. 1991 Aug;12(8):256-7 PMID: 1680337
  31. Cytokines: is there biological meaning?
    Curr Opin Immunol. 1991 Jun;3(3):311-4 PMID: 1910608
  32. Antigen specificity of gamma delta T lymphocytes.
    FASEB J. 1991 Sep;5(12):2699-705 PMID: 1717333
  33. The role of T-cell subsets and cytokines in the regulation of infection.
    Immunol Today. 1991 Oct;12(10):346-8 PMID: 1683536
  34. Role of Th-1 lymphocytes in the development of protective immunity against Mycobacterium leprae. Analysis of lymphocyte function by polymerase chain reaction detection of cytokine messenger RNA.
    J Immunol. 1992 Mar 15;148(6):1885-9 PMID: 1531845
  35. Preferential messenger RNA expression of Th1-type cells (IFN-gamma+, IL-2+) in classical delayed-type (tuberculin) hypersensitivity reactions in human skin.
    J Immunol. 1992 Apr 1;148(7):2058-61 PMID: 1531996
  36. Reciprocal regulatory effects of IFN-gamma and IL-4 on the in vitro development of human Th1 and Th2 clones.
    J Immunol. 1992 Apr 1;148(7):2142-7 PMID: 1532000
  37. Evaluation of the clinical usefulness of mycobacterial skin test antigens in adults with pulmonary mycobacterioses.
    Am Rev Respir Dis. 1992 May;145(5):1160-6 PMID: 1586061
  38. Spleen cell cytokine secretion in Mycobacterium bovis BCG-infected mice.
    Infect Immun. 1992 Jul;60(7):2880-6 PMID: 1612754
  39. Cytokine patterns of immunologically mediated tissue damage.
    J Immunol. 1992 Aug 15;149(4):1470-5 PMID: 1500726
  40. Analysis of cytokine production by Mycobacterium-reactive T cells. Failure to explain Mycobacterium leprae-specific nonresponsiveness of peripheral blood T cells from lepromatous leprosy patients.
    J Immunol. 1993 May 15;150(10):4641-51 PMID: 8482851
  41. Striking the right balance; the role of cytokines in mycobacterial disease.
    Clin Exp Immunol. 1993 Oct;94(1):1-3 PMID: 8403490
  42. Number of interleukin-4- and interferon-gamma-secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutinin: distinct response profiles depending on the type of antigen used for activation.
    Eur J Immunol. 1993 Nov;23(11):2740-5 PMID: 8223849
  43. Cytokine gene expression by cultures of human lymphocytes with autologous Mycobacterium tuberculosis-infected monocytes.
    Infect Immun. 1994 Apr;62(4):1444-50 PMID: 8132351
  44. Preliminary evidence for the trapping of antigen-specific lymphocytes in the lymphoid tissue of 'anergic' tuberculosis patients.
    Clin Exp Immunol. 1976 Oct;26(1):129-32 PMID: 826362
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.62.12.5673-5678.1994
Published
1994-12-00
Pages
5673-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC303319
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.62.12.5673-5678.1994 PMC Full Text View journal details
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