Characterization of the Thermus thermophilus locus encoding peptide deformylase and methionyl-tRNA(fMet) formyltransferase.

Journal of bacteriology ·Vol. 176 ·No. 23 ·1994-12-00 ·Pages 7387-90

Meinnel T, Blanquet S

Abstract

An Escherichia coli strain with thermosensitive expression of the gene encoding peptide deformylase (fms) has been constructed. At nonpermissive temperatures, this strain fails to grow. The essential character of the fms gene was further used to clone by heterologous complementation the locus corresponding to Thermus thermophilus peptide deformylase. The cloned fragment also carries the methionyl-tRNA(fMet) formyltransferase gene (fmt). It is located immediately downstream from the fms gene, as in E. coli. Further sequence analysis of the region surrounding the E. coli fms-fmt locus indicates that the genes bordering the fms-fmt region are not conserved in T. thermophilus.

Related Genes

fms fmt trkA

MeSH Terms

Acyltransferases/genetics Amidohydrolases Amino Acid Sequence Aminopeptidases/genetics Carrier Proteins/genetics Cloning, Molecular Escherichia coli/genetics Genes, Bacterial/genetics Genes, Lethal Hydroxymethyl and Formyl Transferases Membrane Proteins/genetics Molecular Sequence Data Mutation Receptor, trkA Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid Thermus thermophilus/genetics

Chemicals

Carrier Proteins Membrane Proteins Hydroxymethyl and Formyl Transferases methionyl-tRNA formyltransferase Acyltransferases Receptor, trkA Aminopeptidases Amidohydrolases peptide deformylase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Meinnel T

Laboratoire de Biochimie, Unité de Recherche Associée no. 240 du Centre National de la Recherche Scientifique, Ecole Polytechnique, Palaiseau, France.

Blanquet S

References (23)

23 references, click to expand

Construction of an Hfr strain useful for transferring recA mutations between Escherichia coli strains.
J Bacteriol. 1980 Jul;143(1):529-30 PMID: 6249792
Deformylation and protein biosynthesis.
Biochemistry. 1969 Jan;8(1):435-43 PMID: 4887858
Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli.
Nucleic Acids Res. 1985 Jun 11;13(11):3891-903 PMID: 2989779
The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
Improved tools for biological sequence comparison.
Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
Genetic engineering of methionyl-tRNA synthetase: in vitro regeneration of an active synthetase by proteolytic cleavage of a methionyl-tRNA synthetase--beta-galactosidase chimeric protein.
Biochimie. 1988 Jun;70(6):773-82 PMID: 3139093
A unique signature identifies a family of zinc-dependent metallopeptidases.
FEBS Lett. 1989 Jan 2;242(2):211-4 PMID: 2914602
Methionine aminopeptidase gene of Escherichia coli is essential for cell growth.
J Bacteriol. 1989 Jul;171(7):4071-2 PMID: 2544569
New method for generating deletions and gene replacements in Escherichia coli.
J Bacteriol. 1989 Sep;171(9):4617-22 PMID: 2548993
pepM is an essential gene in Salmonella typhimurium.
J Bacteriol. 1989 Sep;171(9):5215-7 PMID: 2670909
Mutations in genes downstream of the rpoN gene (encoding sigma 54) of Klebsiella pneumoniae affect expression from sigma 54-dependent promoters.
Mol Microbiol. 1989 Dec;3(12):1765-75 PMID: 2695747
Zinc coordination, function, and structure of zinc enzymes and other proteins.
Biochemistry. 1990 Jun 19;29(24):5647-59 PMID: 2200508
BISANCE: a French service for access to biomolecular sequence databases.
Comput Appl Biosci. 1990 Oct;6(4):355-6 PMID: 2257496
Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli.
J Bacteriol. 1992 Jul;174(13):4294-301 PMID: 1624424
The Escherichia coli fmt gene, encoding methionyl-tRNA(fMet) formyltransferase, escapes metabolic control.
J Bacteriol. 1993 Feb;175(4):993-1000 PMID: 8432722
Evidence for a novel glycinamide ribonucleotide transformylase in Escherichia coli.
J Bacteriol. 1993 Jun;175(11):3591-7 PMID: 8501063
purU, a source of formate for purT-dependent phosphoribosyl-N-formylglycinamide synthesis.
J Bacteriol. 1993 Nov;175(21):7066-73 PMID: 8226647
Evidence that peptide deformylase and methionyl-tRNA(fMet) formyltransferase are encoded within the same operon in Escherichia coli.
J Bacteriol. 1993 Dec;175(23):7737-40 PMID: 8244948
Genetic characterization of polypeptide deformylase, a distinctive enzyme of eubacterial translation.
EMBO J. 1994 Feb 15;13(4):914-23 PMID: 8112305
Methionine as translation start signal: a review of the enzymes of the pathway in Escherichia coli.
Biochimie. 1993;75(12):1061-75 PMID: 8199241
Protein chain initiation and deformylation in B. subtilis homogenates.
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1487-94 PMID: 4970729
On the release of the formyl group from nascent protein.
J Mol Biol. 1968 May 14;33(3):571-89 PMID: 4973445
An E. coli gene product required for lambda site-specific recombination.
Cell. 1980 Jul;20(3):711-9 PMID: 6251971

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.176.23.7387-7390.1994

Published

1994-12-00

Pages

7387-90

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC197131

Subset

Databases

GENBANK

X77091, X79087

External Links

PubMed source DOI: 10.1128/jb.176.23.7387-7390.1994 PMC Full Text View journal details

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Characterization of the Thermus thermophilus locus encoding peptide deformylase and methionyl-tRNA(fMet) formyltransferase.

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