Home LiteratureArticle Details
PMID: 796674 Published · ppublish English Journal Article

Gal mRNA initiated within IS2.

Molecular & general genetics : MGG ·Vol. 149 ·No. 2 ·1976-12-08 ·Pages 135-43

Rak B

Abstract

A strain of E. coli carrying IS2 in the control region of the gal operon has been found to revert to a constitutive phenotype. These revertants can be divided into two classes, which differ in their rate of enzyme synthesis and gal transcription. One revertant synthesizes the gal messenger at a low level (low level revertants), the other at a higher level that exceeds that of the induced wild type two- to three-fold (high level revertants). In both cases it has been shown that the gal messenger is covalently bound to IS2-RNA, which is transcribed from IS2 in the original orientation. The evidence suggests, that the section of the IS2-element, from which this RNA is transcribed in the case of the high level revertants is smaller than 50 nucleotides long: i.e. the resolution of the hybridization method used for the detection of sequence homologies exceeds that of the electron microscopical heteroduplex technique.

MeSH Terms
Coliphages DNA, Viral Escherichia coli Galactose/metabolism Lysogeny Mutation Nucleic Acid Hybridization Operon Plasmids RNA, Bacterial RNA, Messenger RNA, Viral Transcription, Genetic
Chemicals
DNA, Viral RNA, Bacterial RNA, Messenger RNA, Viral Galactose
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Rak B
References (22)
22 references, click to expand
  1. Integrative and excisive recombination by bacteriophage lambda: evidence for an excision-specific recombination protein.
    J Mol Biol. 1970 Feb 14;47(3):575-83 PMID: 4907273
  2. Mutations of coliphage lambda affecting the expression of replicative functions O and P.
    Mol Gen Genet. 1970;108(3):266-76 PMID: 5475574
  3. Fractionation of the complementary strands of coliphage lambda DNA based on the asymmetric distribution of the poly I,G-binding sites.
    Virology. 1967 Aug;32(4):633-43 PMID: 18614063
  4. mRNA distal to polar nonsense and insertion mutation in the gal operon of E. coli.
    Mol Gen Genet. 1973 May 9;122(3):279-86 PMID: 4577901
  5. Insertion mutations in the control region of the galactose operon of E. coli. II. Physical characterization of the mutations.
    Mol Gen Genet. 1972;115(3):266-76 PMID: 4555680
  6. Relief of polarity in DNA-dependent cell-free synthesis of enzymes of the galactose operon of Escherichia coli.
    Mol Gen Genet. 1972;116(3):258-76 PMID: 4558646
  7. Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientations of their long and short components.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4243-7 PMID: 172900
  8. New features of the regulation of the tryptophan operon.
    Science. 1975 Jul 4;189(4196):22-6 PMID: 1094538
  9. Insertion mutations in the control region of the Gal operon of E. coli. I. Biological characterization of the mutations.
    Mol Gen Genet. 1972;115(3):258-65 PMID: 4555679
  10. Fusions of the lac and trp regions of escherichia coli: covalently fused messenger RNA.
    J Mol Biol. 1971 Aug 28;60(1):203-9 PMID: 4937192
  11. Mechanism of reversion of the gal3 mutation of Escherichia coli.
    Mol Gen Genet. 1975;136(3):243-53 PMID: 16094975
  12. Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12.
    Mol Gen Genet. 1973 May 9;122(3):267-77 PMID: 4577900
  13. Two kinds of insertions in bacterial genes.
    Mol Gen Genet. 1972;119(3):191-206 PMID: 4567154
  14. Non-random distribution of deletion endpoints in the gal operon of E. coli.
    Mol Gen Genet. 1974;132(3):203-13 PMID: 4608964
  15. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.
    J Mol Biol. 1972 Nov 14;71(2):471-97 PMID: 4564488
  16. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
    Virology. 1970 Mar;40(3):734-44 PMID: 4908735
  17. The enzymes of the galactose operon in Escherichia coli. IV. The frequencies of translation of the terminal cistrons in the operon.
    J Biol Chem. 1969 Apr 25;244(8):2143-8 PMID: 4889466
  18. Two insertions in the galactose operon having different sizes but homologous DNA sequences.
    Mol Gen Genet. 1969 Aug 15;104(4):371-7 PMID: 4904509
  19. Structure of inserted bacteriophage Mu-1 DNA and physical mapping of bacterial genes by Mu-1 DNA insertion.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2823-7 PMID: 4562742
  20. 0 degree mutations in the galactose operon in E. coli. I. Genetic characterization.
    Mol Gen Genet. 1967;100(2):178-89 PMID: 4869989
  21. IS2, a genetic element for turn-off and turn-on of gene activity in E. coli.
    Mol Gen Genet. 1974;132(4):265-89 PMID: 4610339
  22. Isolation of pure lac operon DNA.
    Nature. 1969 Nov 22;224(5221):768-74 PMID: 4902927
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332881
Published
1976-12-08
Pages
135-43
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332881
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2