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PMID: 7971177 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of internal chloride on ATP-sensitive K-channels in mouse pancreatic beta-cells.

Pflugers Archiv : European journal of physiology ·Vol. 428 ·No. 2 ·1994-09-00 ·Pages 194-6

Takano M, Ashcroft FM

Abstract

The effect of internal Cl- on the K-ATP channel of pancreatic beta-cells was examined. Reducing Cl- from 140mM to 14mM potentiated channel activity (NPO) approximately 4.5 fold in 60% of patches but was without effect in 40% of patches. The Ki for channel inhibition by ATP was not changed. The inhibitory effect of Cl- interacted with the stimulatory action of MgGDP. In 140mM Cl-, 1mM MgGDP increased NPO in Cl(-)-sensitive patches only: when NPO was potentiated by 14mM Cl-, no further increase was produced by MgGDP. These observations suggest that MgGDP and low Cl- solution may increase channel activity via a similar mechanism.

MeSH Terms

Adenosine Triphosphate/metabolism Animals Cells, Cultured Chlorides/pharmacology Intracellular Membranes/drug effects,metabolism Islets of Langerhans/drug effects,metabolism Membrane Potentials Mice Patch-Clamp Techniques Potassium Channels/drug effects,metabolism

Chemicals

Chlorides Potassium Channels Adenosine Triphosphate

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Takano M

University Laboratory of Physiology, Oxford, UK.

Ashcroft F M

References (13)

13 references, click to expand

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Article Info

Journal

Pflugers Archiv : European journal of physiology

Abbr.

Pflugers Arch

ISSN

0031-6768

DOI

10.1007/BF00374859

Published

1994-09-00

Pages

194-6

Language

English

Region

Germany

NLM ID

0154720

Subset

Grants

Wellcome Trust · United Kingdom

External Links

PubMed source DOI: 10.1007/BF00374859 View journal details

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Effects of internal chloride on ATP-sensitive K-channels in mouse pancreatic beta-cells.

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