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PMID: 7972053 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Spermatogenesis following male germ-cell transplantation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 24 ·1994-11-22 ·Pages 11298-302

Brinster RL, Zimmermann JW

Abstract

In the adult male, a population of diploid stem-cell spermatogonia continuously undergoes self-renewal and produces progeny cells, which initiate the complex process of cellular differentiation that results in mature spermatozoa. We report here that stem cells isolated from testes of donor male mice will repopulate sterile testes when injected into seminiferous tubules. Donor cell spermatogenesis in recipient testes showed normal morpholigical characteristics and produced mature spermatozoa. This methodology, besides opening new avenues of basic research into spermatogenesis and stem-cell self-renewal, may prove useful as a tool for biomedical science and biotechnology.

MeSH Terms
Animals Busulfan Infertility, Male/pathology Male Mice Mice, Inbred C57BL Mice, Inbred Strains Microinjections Seminiferous Tubules/cytology Spermatogenesis Spermatogonia/transplantation Stem Cell Transplantation Testis/cytology
Chemicals
Busulfan
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brinster R L
Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
Zimmermann J W
References (21)
21 references, click to expand
  1. Effects of busulfan on murine spermatogenesis: cytotoxicity, sterility, sperm abnormalities, and dominant lethal mutations.
    Mutat Res. 1987 Feb;176(2):259-68 PMID: 3807936
  2. Regulation of testicular function: a spatial and temporal view.
    Int Rev Physiol. 1980;22:41-115 PMID: 6248479
  3. Transgenic animals.
    Science. 1988 Jun 10;240(4858):1468-74 PMID: 3287623
  4. Genetic engineering of livestock.
    Science. 1989 Jun 16;244(4910):1281-8 PMID: 2499927
  5. Altering the genome by homologous recombination.
    Science. 1989 Jun 16;244(4910):1288-92 PMID: 2660260
  6. Genetic manipulation of mammals and its application in reproductive biology.
    J Reprod Fertil. 1991 Jul;92(2):245-79 PMID: 1886087
  7. The dopamine beta-hydroxylase gene promoter directs expression of E. coli lacZ to sympathetic and other neurons in adult transgenic mice.
    Neuron. 1991 Nov;7(5):703-16 PMID: 1742021
  8. Animal models of human genetic diseases.
    Trends Genet. 1993 Apr;9(4):112-6 PMID: 8516844
  9. Developmentally regulated expression of a mouse germ cell nuclear antigen examined from embryonic day 11 to adult in male and female mice.
    Dev Biol. 1994 Jun;163(2):331-40 PMID: 8200475
  10. Generation of transgenic dairy cattle from transgene-analyzed and sexed embryos produced in vitro.
    Biotechnology (N Y). 1994 Jun;12(6):606-8 PMID: 7764950
  11. Expression of a mouse Zfy-1/lacZ transgene in the somatic cells of the embryonic gonad and germ cells of the adult testis.
    Development. 1994 Jun;120(6):1549-59 PMID: 8050362
  12. Germline transmission of donor haplotype following spermatogonial transplantation.
    Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11303-7 PMID: 7972054
  13. Introduction of genes into the germ line of animals.
    Harvey Lect. 1984-1985;80:1-38 PMID: 6400653
  14. Radiation survival and regeneration characteristics of spermatogenic stem cells of mouse testis.
    Radiat Res. 1974 Jan;57(1):88-103 PMID: 10874929
  15. Re-examination of spermatogonial renewal in the rat by means of seminiferous tubules mounted "in toto".
    Am J Anat. 1968 Mar;122(2):237-47 PMID: 5659131
  16. Role of spermatogonia in the repair of the seminiferous epithelium following x-irradiation of the rat testis.
    Am J Anat. 1970 Jul;128(3):265-82 PMID: 4193812
  17. The spermatogonial stem cell population in adult rats. I. Their morphology, proliferation and maturation.
    Anat Rec. 1971 Mar;169(3):533-57 PMID: 5550532
  18. Induced chimaerism by transplanting embryonic cells into mouse blastocysts.
    J Exp Zool. 1972 Aug;181(2):193-201 PMID: 5047360
  19. Spermatogenetic clones developing from repopulating stem cells surviving a high dose of an alkylating agent.
    Cell Tissue Kinet. 1975 Nov;8(6):543-51 PMID: 1238171
  20. Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization.
    J Cell Biol. 1977 Jul;74(1):68-85 PMID: 874003
  21. Sertoli cell differentiation in the testes of mice genetically deficient in germ cells.
    Biol Reprod. 1979 Jun;20(5):1031-8 PMID: 476239
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.24.11298
Published
1994-11-22
Pages
11298-302
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC45218
Subset
IM
Grants
NICHD NIH HHS · HD23657 · United States
Corrections
CommentIn
PMID: 7972051
External Links
PubMed source DOI: 10.1073/pnas.91.24.11298 PMC Full Text View journal details
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