A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes.

The Biochemical journal ·Vol. 160 ·No. 3 ·1976-12-15 ·Pages 505-19

Cox RA, Hirst W

Abstract

Mg2+ was shown to affect the conformation of rRNA over the range of 0.03-1.2M-KCl. The species studies were Escherichia coli S-rRNA and L-rRNA (the RNA moieties of the smaller and larger subribosomal particles respectively) and rabbits S-rRNA and L-rRNA. 2. The addition of Mg2+ to rRNA in reconstitution buffer (0.35M-KCl0.01M-Tris/HCl, pH7.2) at 20 degrees C let to an increase in bihelical secondary structure through the formation of additional (mainly A-U) base-pairs (e.g. an additional approx. 58 A-U base-pairs per molecule of E. coli S-rRNA as judged by u.v. difference spectrophotometry...

MeSH Terms

Ammonium Chloride/pharmacology Animals Base Sequence Escherichia coli/ultrastructure Magnesium/pharmacology Nucleic Acid Conformation/drug effects Potassium Chloride/pharmacology RNA, Ribosomal Rabbits Reticulocytes/ultrastructure Ribosomes/drug effects Spectrophotometry, Ultraviolet

Chemicals

RNA, Ribosomal Ammonium Chloride Potassium Chloride Magnesium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cox R A

Hirst W

References (25)

25 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1600505

Published

1976-12-15

Pages

505-19

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1164267

Subset

External Links

PubMed source DOI: 10.1042/bj1600505 PMC Full Text View journal details

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A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes.

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