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PMID: 7986055 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A transcriptional reporter for in vivo promoter analysis in the archaeon Haloferax volcanii.

Applied and environmental microbiology ·Vol. 60 ·No. 10 ·1994-10-00 ·Pages 3867-9

Palmer JR, Daniels CJ

Abstract

We have used a modified intron-containing tRNA(Pro(UGG) gene (tRNA(ProM), derived from the Saccharomyces cerevisiae tRNA(Pro(UGG) gene, as a reporter to measure in vivo transcription from a halophilic archaeon promoter. Coupling of the yeast tRNA(ProM) gene to the Haloferax volcanii tRNA(Lys) promoter on the H. volcanii plasmid pWL201 led to the production of a single stable transcript that was readily quantitated by Northern (RNA) blot analysis. Comparison of tRNA(ProM) RNA production from constructs containing the wild-type tRNA(Lys) promoter and those containing mutant tRNA(Lys) promoters demonstrated that this assay system can be used to measure expression from strong and weak promoters.

MeSH Terms
Base Sequence DNA, Bacterial/genetics Genes, Bacterial Genes, Reporter Halobacteriales/genetics Molecular Sequence Data Promoter Regions, Genetic RNA, Transfer, Pro/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA, Bacterial RNA, Transfer, Pro
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Palmer J R
Department of Microbiology, Ohio State University, Columbus 43210.
Daniels C J
References (16)
16 references, click to expand
  1. Mutational analysis of an archaebacterial promoter: essential role of a TATA box for transcription efficiency and start-site selection in vitro.
    Proc Natl Acad Sci U S A. 1990 Dec;87(24):9509-13 PMID: 2124695
  2. An expression vector for the archaebacterium Haloferax volcanii.
    J Bacteriol. 1990 Dec;172(12):7104-10 PMID: 1701429
  3. The RNA component of RNase P from the archaebacterium Haloferax volcanii.
    J Biol Chem. 1991 Mar 25;266(9):5689-95 PMID: 1706337
  4. Control regions of an archaeal gene. A TATA box and an initiator element promote cell-free transcription of the tRNA(Val) gene of Methanococcus vannielii.
    J Mol Biol. 1991 Dec 5;222(3):495-508 PMID: 1748992
  5. PCR amplification of long DNA fragments.
    Nucleic Acids Res. 1992 Feb 11;20(3):623 PMID: 1741307
  6. TFIIB, an evolutionary link between the transcription machineries of archaebacteria and eukaryotes.
    Cell. 1992 Oct 16;71(2):189-90 PMID: 1423586
  7. Elements of an archaeal promoter defined by mutational analysis.
    Nucleic Acids Res. 1992 Oct 25;20(20):5423-8 PMID: 1279520
  8. Putative tfIIs gene of Sulfolobus acidocaldarius encoding an archaeal transcription elongation factor is situated directly downstream of the gene for a small subunit of DNA-dependent RNA polymerase.
    Nucleic Acids Res. 1993 May 11;21(9):2251 PMID: 8502569
  9. Complete nucleotide sequence of an archaeal (Pyrococcus woesei) gene encoding a homolog of eukaryotic transcription factor IIB (TFIIB).
    Nucleic Acids Res. 1993 Jun 25;21(12):2942 PMID: 8332505
  10. The TATA-binding protein: a general transcription factor in eukaryotes and archaebacteria.
    Science. 1994 May 27;264(5163):1326-9 PMID: 8191287
  11. Expression of a yeast intron-containing tRNA in the archaeon Haloferax volcanii.
    J Bacteriol. 1994 Jun;176(12):3820-3 PMID: 8206862
  12. Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8530-4 PMID: 2825193
  13. Comparative evaluation of gene expression in archaebacteria.
    Eur J Biochem. 1988 May 2;173(3):473-82 PMID: 3131139
  14. Transformation methods for halophilic archaebacteria.
    Can J Microbiol. 1989 Jan;35(1):148-52 PMID: 2497937
  15. Shuttle vectors for the archaebacterium Halobacterium volcanii.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5478-82 PMID: 2748598
  16. Mutations in DNA gyrase result in novobiocin resistance in halophilic archaebacteria.
    J Bacteriol. 1991 Jan;173(2):642-8 PMID: 1846146
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.60.10.3867-3869.1994
Published
1994-10-00
Pages
3867-9
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC201898
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.60.10.3867-3869.1994 PMC Full Text View journal details
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