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PMID: 799251 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transfection of Escherichia coli by Mu DNA.

Molecular & general genetics : MGG ·Vol. 149 ·No. 3 ·1976-12-22 ·Pages 323-8

Kahmann R, Kamp D, Zipser D

Abstract

Infectivity of Mu DNA was demonstrated in Ca+ +-treated Escherichia coli cells that lacked the nucleases Exo V and Endo I. The efficiency of transfection is about 10(-7) per phage equivalent. Infectivity is destroyed by denaturation of Mu DNA, and cannot be restored by renaturation.

MeSH Terms
Calcium/pharmacology Coliphages/drug effects,metabolism DNA, Viral/metabolism Escherichia coli/drug effects,metabolism Genotype Kinetics Phenotype Species Specificity Transformation, Genetic Virus Replication
Chemicals
DNA, Viral Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kahmann R
Kamp D
Zipser D
References (36)
36 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268534
Published
1976-12-22
Pages
323-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00268534
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