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PMID: 7998930 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Insulin receptor substrate 1 is phosphorylated by the serine kinase activity of phosphatidylinositol 3-kinase.

The Biochemical journal ·Vol. 304 ( Pt 1) ·1994-11-15 ·Pages 17-21

Tanti JF, Grémeaux T, Van Obberghen E, Le Marchand-Brustel Y

Abstract

Insulin receptor substrate (IRS) 1, which is tyrosine phosphorylated in response to insulin, presents multiple serine/threonine phosphorylation sites. To search for a serine kinase activity towards IRS 1, immunoprecipitates from basal or stimulated 3T3-L1 adipocytes were used in an in vitro kinase assay. When IRS 1 was isolated from insulin-treated cells, serine phosphorylation of IRS 1 occurred, which we attribute to the kinase activity of the phosphatidylinositol 3-kinase (PI3-kinase). Importantly, in an in vitro reconstitution assay, an excess of the PI3-kinase subunit prevents this phosphorylation. Together, our results suggest that following insulin stimulation, PI3-kinase associates with IRS 1, allowing for its serine phosphorylation. This phosphorylation event could play a role in the modulation of insulin signalling.

MeSH Terms
3T3 Cells Animals Enzyme Activation Mice Phosphatidylinositol 3-Kinases Phosphorylation Phosphotransferases (Alcohol Group Acceptor)/metabolism Protein Serine-Threonine Kinases/metabolism Receptor, Insulin/metabolism Substrate Specificity
Chemicals
Phosphotransferases (Alcohol Group Acceptor) Receptor, Insulin Protein Serine-Threonine Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tanti J F
Institut National de la Santé et de la Recherche Médicale, INSERM U 145, Faculté de Médecine, Nice, France.
Grémeaux T
Van Obberghen E
Le Marchand-Brustel Y
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3040017
Published
1994-11-15
Pages
17-21
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1137444
Subset
IM
External Links
PubMed source DOI: 10.1042/bj3040017 PMC Full Text View journal details
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