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PMID: 8001134 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Mutation of a meiosis-specific MutS homolog decreases crossing over but not mismatch correction.

Cell ·Vol. 79 ·No. 6 ·1994-12-16 ·Pages 1069-80

Ross-Macdonald P, Roeder GS

Abstract

MSH4 is a novel meiosis-specific gene required for wild-type levels of spore viability in S. cerevisiae. The predicted product of the MSH4 gene is homologous to the MutS family of proteins; however, msh4-null mutants have no apparent defect in mismatch repair. msh4 mutant strains display wild-type levels of gene conversion and postmeiotic segregation, but they show a reduction in crossing over and a resultant increase in nondisjunction of homologous chromosomes at meiosis I. Immunofluorescence experiments demonstrate that the Msh4 protein is localized to discrete sites on pachytene chromosomes. We propose that Msh4 interacts with a recombination intermediate to influence its resolution.

MeSH Terms

Adenosine Triphosphatases Amino Acid Sequence Bacterial Proteins/classification,genetics Base Sequence Chromosomes, Fungal/chemistry,ultrastructure Crossing Over, Genetic/genetics DNA Repair/genetics DNA-Binding Proteins Escherichia coli Proteins Fungal Proteins/chemistry,genetics,isolation & purification Gene Conversion/genetics Genes, Fungal/genetics Immunohistochemistry Meiosis/genetics Molecular Sequence Data Multigene Family/genetics MutS DNA Mismatch-Binding Protein Mutagenesis Nondisjunction, Genetic Polymerase Chain Reaction Recombination, Genetic/genetics Saccharomyces cerevisiae/genetics,growth & development Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Spores, Fungal/genetics,growth & development

Chemicals

Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins Fungal Proteins MSH4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Adenosine Triphosphatases MutS DNA Mismatch-Binding Protein MutS protein, E coli

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ross-Macdonald P

Department of Biology, Yale University, New Haven, Connecticut 06520-8103.

Roeder G S

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/0092-8674(94)90037-x

Published

1994-12-16

Pages

1069-80

Language

English

Region

United States

NLM ID

0413066

Subset

Grants

NIGMS NIH HHS · GM28904 · United States

Databases

GENBANK

U13999

External Links

PubMed source DOI: 10.1016/0092-8674(94)90037-x View journal details

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Mutation of a meiosis-specific MutS homolog decreases crossing over but not mismatch correction.

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