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PMID: 8001165 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A luciferase expression system for Physarum that facilitates analysis of regulatory elements.

Current genetics ·Vol. 26 ·No. 2 ·1994-08-00 ·Pages 126-31

Bailey J, Benard M, Burland TG

Abstract

We have developed a transient expression system for the protist Physarum polycephalum based on firefly luciferase. We demonstrate the utility of this system for comparing the activities of different promoters in Physarum amoebae, and also for detecting genetic elements that affect the level of gene expression. This system is likely to facilitate improvements in the stable transformation of this organism.

MeSH Terms
Animals Cloning, Molecular/methods Coleoptera Electroporation Gene Expression Kinetics Luciferases/biosynthesis,genetics,metabolism Physarum polycephalum/metabolism Promoter Regions, Genetic Recombinant Proteins/biosynthesis,metabolism Restriction Mapping
Chemicals
Recombinant Proteins Luciferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bailey J
Genetics Department, University of Leicester, UK.
Benard M
Burland T G
References (17)
17 references, click to expand
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00313799
Published
1994-08-00
Pages
126-31
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Grants
NCI NIH HHS · CA07175 · United States
NCI NIH HHS · CA23076 · United States
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1007/BF00313799 View journal details
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