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PMID: 8018871 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential accumulation of S-adenosylmethionine synthetase transcripts in response to salt stress.

Plant molecular biology ·Vol. 25 ·No. 2 ·1994-05-00 ·Pages 217-27

Espartero J, Pintor-Toro JA, Pardo JM

Abstract

NaCl stress causes the accumulation of several mRNAs in tomato seedlings. An upregulated cDNA clone, SAM1, was found to encode a S-adenosyl-L-methionine synthetase enzyme (AdoMet synthetase). Expression of the cDNA SAM1 in a yeast mutant lacking functional SAM genes resulted in high AdoMet synthetase activity and AdoMet accumulation. We show that tomato plants contain at least four SAM isogenes. Clones corresponding to isogenes SAM2 and SAM3 have also been isolated and sequenced. They encode predicted polypeptides 95% and 92% identical, respectively, to the SAM1-encoded AdoMet Synthetase. RNA hybridization analysis showed a differential response of SAM genes to salt and other stress treatments. SAM1 and SAM3 mRNAs accumulated in the root in response to NaCl, mannitol or ABA treatments. SAM1 mRNA accumulated also in leaf tissue. These increases of mRNA level were apparent as soon as 8 h after the initiation of the salt treatment and were maintained for at least 3 days. A possible role for AdoMet synthetases in the adaptation to salt stress is discussed.

Related Genes
SAM1 SAM2 SAM3
MeSH Terms
Abscisic Acid/pharmacology Amino Acid Sequence Blotting, Northern Blotting, Southern DNA Probes Genes, Plant Isoenzymes/chemistry,genetics Mannitol/pharmacology Methionine Adenosyltransferase/chemistry,genetics Molecular Sequence Data Multigene Family RNA, Messenger/genetics,metabolism Sodium Chloride/pharmacology Vegetables/drug effects,enzymology,genetics
Chemicals
DNA Probes Isoenzymes RNA, Messenger Mannitol Sodium Chloride Abscisic Acid Methionine Adenosyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Espartero J
Instituto de Recursos Naturales y Agrobiología, C.S.I.C., Sevilla, Spain.
Pintor-Toro J A
Pardo J M
References (40)
40 references, click to expand
  1. Induction by fungal elicitor of S-adenosyl-L-methionine synthetase and S-adenosyl-L-homocysteine hydrolase mRNAs in cultured cells and leaves of Petroselinum crispum.
    Proc Natl Acad Sci U S A. 1992 May 15;89(10):4713-7 PMID: 1374911
  2. Yeast/E. coli shuttle vectors with multiple unique restriction sites.
    Yeast. 1986 Sep;2(3):163-7 PMID: 3333305
  3. Structural and transcriptional characterization of the external spacer of a ribosomal RNA nuclear gene from a higher plant.
    Eur J Biochem. 1988 Mar 15;172(3):767-76 PMID: 3350023
  4. Molecular cloning and nucleotide sequence of cDNA encoding the rat kidney S-adenosylmethionine synthetase.
    J Biol Chem. 1990 Aug 15;265(23):13683-6 PMID: 1696256
  5. A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants.
    Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9354-8 PMID: 1384052
  6. S-adenosyl methionine-mediated repression of methionine biosynthetic enzymes in Saccharomyces cerevisiae.
    J Bacteriol. 1973 Jun;114(3):928-33 PMID: 4576408
  7. Molecular cloning and nucleotide sequence of cDNA encoding the human liver S-adenosylmethionine synthetase.
    Biochem Int. 1991 Sep;25(1):81-90 PMID: 1772450
  8. Messenger RNA for G1 protein of French bean seeds: Cell-free translation and product characterization.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3196-200 PMID: 16592543
  9. SAM2 encodes the second methionine S-adenosyl transferase in Saccharomyces cerevisiae: physiology and regulation of both enzymes.
    Mol Cell Biol. 1988 Dec;8(12):5132-9 PMID: 3072475
  10. Proteins Associated with Adaptation of Cultured Tobacco Cells to NaCl.
    Plant Physiol. 1985 Sep;79(1):126-37 PMID: 16664357
  11. Cell wall proteins at low water potentials.
    Plant Physiol. 1987 Sep;85(1):261-7 PMID: 16665667
  12. Effects of Salinity on Water Transport of Excised Maize (Zea mays L.) Roots.
    Plant Physiol. 1991 Nov;97(3):1136-45 PMID: 16668500
  13. The synthesis of the two S-adenosyl-methionine synthetases is differently regulated in Saccharomyces cerevisiae.
    Mol Gen Genet. 1991 Apr;226(1-2):224-32 PMID: 1903502
  14. Wall extensibility and cell hydraulic conductivity decrease in enlarging stem tissues at low water potentials.
    Plant Physiol. 1990 Aug;93(4):1610-9 PMID: 16667664
  15. Structure and expression analyses of the S-adenosylmethionine synthetase gene family in Arabidopsis thaliana.
    Gene. 1989 Dec 14;84(2):359-69 PMID: 2482229
  16. Water deficit modulates gene expression in growing zones of soybean seedlings. Analysis of differentially expressed cDNAs, a new beta-tubulin gene, and expression of genes encoding cell wall proteins.
    Plant Mol Biol. 1991 Oct;17(4):591-608 PMID: 1912487
  17. Stress Responses in Alfalfa (Medicago sativa L.): X. Molecular Cloning and Expression of S-Adenosyl-l-Methionine:Caffeic Acid 3-O-Methyltransferase, a Key Enzyme of Lignin Biosynthesis.
    Plant Physiol. 1991 Sep;97(1):7-14 PMID: 16668418
  18. Gene expression in response to abscisic acid and osmotic stress.
    Plant Cell. 1990 Jun;2(6):503-12 PMID: 2152172
  19. A tomato cDNA inducible by salt stress and abscisic acid: nucleotide sequence and expression pattern.
    Plant Mol Biol. 1990 Nov;15(5):695-705 PMID: 2151719
  20. Differential expression of five Arabidopsis genes encoding glycine-rich proteins.
    Plant Cell. 1990 May;2(5):427-36 PMID: 2152168
  21. Induction of a Putative Ca-ATPase mRNA in NaCl-Adapted Cells.
    Plant Physiol. 1992 Nov;100(3):1471-8 PMID: 16653146
  22. A novel methyl transferase induced by osmotic stress in the facultative halophyte Mesembryanthemum crystallinum.
    EMBO J. 1992 Jun;11(6):2077-85 PMID: 1600940
  23. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  24. Strong cellular preference in the expression of a housekeeping gene of Arabidopsis thaliana encoding S-adenosylmethionine synthetase.
    Plant Cell. 1989 Jan;1(1):81-93 PMID: 2535470
  25. Isolation and characterization of a tomato cDNA clone which codes for a salt-induced protein.
    Plant Mol Biol. 1988 Sep;10(5):401-12 PMID: 24277588
  26. Higher plant Ca(2+)-ATPase: primary structure and regulation of mRNA abundance by salt.
    Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9205-9 PMID: 1384045
  27. The sequence of metK, the structural gene for S-adenosylmethionine synthetase in Escherichia coli.
    J Biol Chem. 1984 Dec 10;259(23):14505-7 PMID: 6094561
  28. A rapid assay procedure for ATP:L-methionine adenosyltransferase.
    Biochim Biophys Acta. 1972 Aug 28;276(2):399-406 PMID: 4560566
  29. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  30. Salt stress leads to differential expression of two isogenes of phosphoenolpyruvate carboxylase during Crassulacean acid metabolism induction in the common ice plant.
    Plant Cell. 1989 Jul;1(7):715-25 PMID: 2535520
  31. Methionine adenosyltransferase (S-adenosylmethionine synthetase) and S-adenosylmethionine decarboxylase.
    Adv Enzymol Relat Areas Mol Biol. 1984;56:251-82 PMID: 6364703
  32. Four major transcriptional responses in the methionine/threonine biosynthetic pathway of Saccharomyces cerevisiae.
    Yeast. 1991 Nov;7(8):781-803 PMID: 1789001
  33. Abscisic acid and water-stress induce the expression of a novel rice gene.
    EMBO J. 1988 Aug;7(8):2279-86 PMID: 2973410
  34. Plant transposable elements generate the DNA sequence diversity needed in evolution.
    EMBO J. 1985 Mar;4(3):591-7 PMID: 15926219
  35. NaCl Regulation of Tonoplast ATPase 70-Kilodalton Subunit mRNA in Tobacco Cells.
    Plant Physiol. 1991 Oct;97(2):562-8 PMID: 16668435
  36. Expression of ethylene biosynthetic pathway transcripts in senescing carnation flowers.
    Plant Physiol. 1992 Jun;99(2):526-32 PMID: 16668918
  37. Structural Changes and Associated Reduction of Hydraulic Conductance in Roots of Sorghum bicolor L. following Exposure to Water Deficit.
    Plant Physiol. 1992 May;99(1):203-12 PMID: 16668850
  38. Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+- and Ca2+-ATPases.
    Nature. 1986 Feb 20-26;319(6055):689-93 PMID: 3005867
  39. Cloning and nucleotide sequence of a s-adenosylmethionine synthetase cDNA from carnation.
    Plant Physiol. 1991 Jul;96(3):997-9 PMID: 16668288
  40. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00023239
Published
1994-05-00
Pages
217-27
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
Z24741, Z24742, Z24743
External Links
PubMed source DOI: 10.1007/BF00023239 View journal details
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