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PMID: 8021222 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular analysis of the rfb gene cluster of a group D2 Salmonella enterica strain: evidence for its origin from an insertion sequence-mediated recombination event between group E and D1 strains.

Journal of bacteriology ·Vol. 176 ·No. 14 ·1994-07-00 ·Pages 4357-65

Xiang SH, Hobbs M, Reeves PR

Abstract

The Salmonella enterica O antigen is a highly variable surface polysaccharide composed of a repeated oligosaccharide (the O unit). The O unit produced by serogroup D2 has structural features in common with those of groups D1 and E1, and hybridization studies had previously suggested that the D2 rfb gene cluster responsible for O-unit biosynthesis is indeed a hybrid of the two. In this study, the rfb gene cluster was cloned from a group D2 strain of S. enterica sv. Strasbourg. Mapping, hybridization, and DNA sequencing showed that the organization of the D2 rfb genes is similar to that of group D1, with the alpha-mannosyl transferase gene rfbU replaced by rfbO, the E1-specific beta-mannosyl transferase gene. The E1-specific polymerase gene (rfc) has also been acquired. Interestingly, the D1-like and E1-like rfb regions are separated by an additional sequence closely related to an element (Hinc repeat [H-rpt]) associated with the Rhs loci of Escherichia coli. The H-rpt resembles an insertion sequence and possibly mediated the intraspecific recombination events which produced the group D2 rfb gene organization.

Related Genes
rfb
MeSH Terms
Amino Acid Sequence Base Sequence Carbohydrate Sequence Cloning, Molecular DNA Transposable Elements DNA, Bacterial/genetics Molecular Sequence Data Multigene Family Recombination, Genetic Salmonella/genetics Sequence Alignment
Chemicals
DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Xiang S H
Department of Microbiology (G08), University of Sydney, New South Wales, Australia.
Hobbs M
Reeves P R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.14.4357-4365.1994
Published
1994-07-00
Pages
4357-65
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205649
Subset
IM
Databases
GENBANK
U04164, U04165
External Links
PubMed source DOI: 10.1128/jb.176.14.4357-4365.1994 PMC Full Text View journal details
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