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PMID: 8022808 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

A role for a eukaryotic GrpE-related protein, Mge1p, in protein translocation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 14 ·1994-07-05 ·Pages 6481-5

Laloraya S, Gambill BD, Craig EA

Abstract

The 70-kDa heat shock proteins (hsp70s) function as molecular chaperones in a wide variety of cellular processes through cycles of binding and release from substrate proteins coupled to cycles of ATP hydrolysis. In the prokaryote Escherichia coli, the hsp70 DnaK functions with two other proteins, DnaJ and GrpE, which modulate the activity of DnaK. While numerous hsp70s and DnaJ-related proteins have been identified in eukaryotes, to our knowledge no GrpE-related proteins have been reported. We report the isolation and characterization of a eukaryotic grpE-related gene, MGE1. MGE1, an essential nuclear gene of the yeast Saccharomyces cerevisiae, encodes a soluble protein of the mitochondrial matrix. Cells with reduced expression of Mge1p accumulate the precursor form of a mitochondrial protein. Since mitochondrial hsp70 is required for translocation of precursors of mitochondrial proteins from the cytosol into the matrix of mitochondria, these data suggest that Mge1p acts in concert with mitochondrial hsp70 in protein translocation.

Related Genes
MGE1 dnaJ dnaK grpE
MeSH Terms
Amino Acid Sequence Bacteria/genetics,metabolism Bacterial Proteins/biosynthesis,metabolism Blotting, Western Carrier Proteins/biosynthesis,metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins Fungal Proteins/biosynthesis,metabolism Genes, Bacterial Genes, Fungal Genotype Heat-Shock Proteins/biosynthesis,metabolism Membrane Transport Proteins Mitochondrial Membrane Transport Proteins Molecular Chaperones Molecular Sequence Data Plasmids Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Subcellular Fractions/metabolism
Chemicals
Bacterial Proteins Carrier Proteins Escherichia coli Proteins Fungal Proteins GrpE protein, Bacteria GrpE protein, E coli Heat-Shock Proteins MGE1 protein, S cerevisiae Membrane Transport Proteins Mitochondrial Membrane Transport Proteins Molecular Chaperones Saccharomyces cerevisiae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Laloraya S
Department of Biomolecular Chemistry, University of Wisconsin-Madison 53706.
Gambill B D
Craig E A
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.14.6481
Published
1994-07-05
Pages
6481-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44226
Subset
IM
Grants
NIGMS NIH HHS · 5 R01 GM31107 · United States
Databases
GENBANK
U09565
External Links
PubMed source DOI: 10.1073/pnas.91.14.6481 PMC Full Text View journal details
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