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PMID: 803474 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evaluation of immunological specificity of flurescein-conjugated antisera with agarose-antigen sections.

Infection and immunity ·Vol. 11 ·No. 2 ·1975-02-00 ·Pages 415-6

Case JB, Lussier LM, Mannik M

Abstract

Purified immunoglobulins were conjugated to agarose beads; the beads were frozen, and used to establish the specificity of fluorescein-conjugated antisera.

MeSH Terms
Animals Antibody Specificity Evaluation Studies as Topic Fluoresceins Fluorescent Antibody Technique Goats/immunology Humans Immune Sera Immunodiffusion/standards Immunoglobulins Polysaccharides Sepharose
Chemicals
Fluoresceins Immune Sera Immunoglobulins Polysaccharides Sepharose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Case J B
Lussier L M
Mannik M
References (7)
7 references, click to expand
  1. IMMUNOFLUORESCENCE APPLIED TO PROTEIN SOLUTIONS.
    J Lab Clin Med. 1963 Sep;62:517-24 PMID: 14065300
  2. The fluorescent antibody technique applied to titration and identification of antigens in solutions or antisera.
    Proc Soc Exp Biol Med. 1963 Jun;113:394-7 PMID: 13941571
  3. An immunofluorescence procedure for the detection of intracellular immunoglobulins.
    Clin Exp Immunol. 1969 Apr;4(4):457-72 PMID: 4182405
  4. The use of polymerized immunoglobulin particles for specificity control of fluorescent conjugates.
    J Immunol Methods. 1974 Mar;4(2):195-202 PMID: 4597105
  5. Microfluorometry on antigen-antibody interaction in immunofluorescence using antigens covalently bound to agarose beads.
    J Immunol Methods. 1973 Jun;2(4):383-92 PMID: 4579446
  6. Covalent binding of molecules to CNBr-activated agarose: parameters relevant to the activation and coupling reactions.
    Biochim Biophys Acta. 1974 Apr 22;343(2):382-91 PMID: 4600124
  7. Antibody-agarose immunoadsorbents: complete removal of classes of immunoglobulins from serum.
    J Immunol. 1971 Jun;106(6):1670-2 PMID: 4103728
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.11.2.415-416.1975
Published
1975-02-00
Pages
415-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415078
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.11.2.415-416.1975 PMC Full Text View journal details
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