The estimation of red cell superoxide dismutase activity.

The Journal of laboratory and clinical medicine ·Vol. 85 ·No. 2 ·1975-02-00 ·Pages 337-41

Winterbourn CC, Hawkins RE, Brian M, Carrell RW

Abstract

A method is described for the estimation of red cell superoxide dismutase (erythrocuprein) and a normal range of activity established. It is likely that this enzyme is essential to the red cell for the detoxification of superoxide radicals, and plays a protective role similar to that of the glutathione-glutathione peroxidase system. It is suggested that superoxide dismutase deficiency may be an unrecognized cause of Heinz body hemolytic anemia. The separation of superoxide dismutase from hemoglobin by polyacrylamide gel electrophoresis is also described. Normal superoxide dismutase activity was measured in one case of Wilson's disease.

MeSH Terms

Adolescent Adult Anemia, Hemolytic/enzymology Animals Blood Preservation Cattle Chloroform Citrates Edetic Acid Electrophoresis, Polyacrylamide Gel Erythrocytes/enzymology Ethanol Female Glucose Hemoglobins/isolation & purification Hemolysis Hepatolenticular Degeneration/blood,enzymology Humans Male Middle Aged Oxidation-Reduction Superoxide Dismutase/blood,deficiency,physiology Tetrazolium Salts

Chemicals

Citrates Hemoglobins Tetrazolium Salts Ethanol Chloroform Edetic Acid Superoxide Dismutase Glucose

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Winterbourn C C

Hawkins R E

Brian M

Carrell R W

Article Info

Journal

The Journal of laboratory and clinical medicine

Abbr.

J Lab Clin Med

ISSN

0022-2143

Published

1975-02-00

Pages

337-41

Language

English

Region

United States

NLM ID

0375375

Subset

External Links

PubMed source

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The estimation of red cell superoxide dismutase activity.

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